The effect of montelukast in a model of gouty arthritis induced by sodium monourate crystals.

Abstract

Non-steroidal anti-inflammatory drugs (NSAIDS) are the first line of therapy in acute gouty arthritis. NSAIDs inhibit the cyclooxygenase pathway, but not the lipooxygenase activity and can have many adverse effects and thus have a limited effect on the control of inflammation in this disease. In this work we studied the effect of montelukast on the cellular inflammatory infiltrate in a model of murine arthritis induced by sodium monourate crystals (SMU), using a subcutaneous air cavity (air pouch) in BALB/c mice. Seven groups of BALB/c mice (n = 4) were distributed into five experimental groups and two inflammatory control groups, a positive and a negative one. Previous to SMU exposure, the experimental groups received montelukast (1 and 0.01 mg/Kg/w) and/or indomethacine (2.5 mg/Kg/w), followed by administration of SMU in the air pouch. The total and differential counts of inflammatory cells were analyzed after 2, 6, 12 and 24 hours. Montelukast, significantly reduced the total number of cells (p < 0.05), with a predominant impact on polymorphonuclear over mononuclear cells, especially after 12 hours of the medication. The montelukast/indometacine combination showed an additive effect. Our data show that montelukast has an anti-inflammatory effect in the model of gouty arthritis. Consequently, anti-leukotrienes could represent a new and effective therapy, either isolated or combined with conventional therapy of gouty arthritis.

• Authors: Loida Ponce, Marjorie Arjona, Gustavo Blanco, Stuart Alvarez, Eduardo Arcila, Arnaldo Ortega, Dubelis Nuñez, Julie Verzura, Robert Tovar, Sarah Bethencourt, Ricardo Riera, Sioly Mora-Orta, José Corado
• Journal: Investigacion clinica (Invest Clin) Vol. 52 Issue 1 Pg. 15-22 (Mar 2011) ISSN: 0535-5133 [Print] Venezuela
• PMID: 21612136 (Publication Type: Journal Article)

Chemical References

• Acetates
• Anti-Inflammatory Agents, Non-Steroidal
• Cyclopropanes
• Leukotriene Antagonists
• Quinolines
• Sulfides
• Uric Acid
• montelukast
• Indomethacin

Topics

• Acetates (administration & dosage, therapeutic use)
• Animals
• Anti-Inflammatory Agents, Non-Steroidal (administration & dosage, therapeutic use)
• Arthritis, Gouty (chemically induced, drug therapy, prevention & control)
• Cell Migration Assays, Leukocyte
• Cyclopropanes
• Disease Models, Animal
• Dose-Response Relationship, Drug
• Drug Evaluation, Preclinical
• Drug Synergism
• Indomethacin (administration & dosage, therapeutic use)
• Inflammation (chemically induced, drug therapy)
• Leukocytes, Mononuclear (drug effects)
• Leukotriene Antagonists (administration & dosage, therapeutic use)
• Male
• Mice
• Mice, Inbred BALB C
• Neutrophils (drug effects)
• Premedication
• Quinolines (administration & dosage, therapeutic use)
• Sulfides
• Uric Acid (toxicity)