[Experimental study of Twist siRNA to inhibit the formation of retinal neovascularization in mice].

Abstract

OBJECTIVE:

The purpose of this research is to find the law of neovascular endothelial cell migration and transition through repressing the expression of Twist in mouse's retinal neovascularization with RNAi, and get a new target of inhibit retinal neovascularization.

METHODS:

Oxygen-induced retinopathy (OIR) was produced in new born C57BL/6J mice by exposing postnatal day 7 (P7) pups to 75% oxygen for 5 days. P12 pups were injected 1 µl pTwist. siRNA plasmid solution or 1 µl negative siRNA plasmid solution into vitreous cavity. Eyeballs were enucleated for Evans blue angiography, histopathologic examination, neovascular endothelial cell counting, immunohistochemistry and Real-Time PCR.

RESULTS:

Observed by light microscopy retinal neovascularization, the number of vascular endothelial cells per eye were 0.34 ± 0.11, 32.73 ± 6.38, 4.56 ± 2.02 and 20.17 ± 6.49 in the normal control group, hyperoxia group, Twist plasmid group and the control plasmid group. Mouse retinal Evans blue perfusion and HE staining of paraffin sections showed that retinal vascular leakage, tortuous and angiogenesis significantly reduced in Twist plasmid group compared with hyperoxia group. Endothelial cell count was significantly decrease in Twist plasmid group. Both immunohistochemistry and real time PCR proved that Twist and vimentin expression in hyperoxia group were significantly higher than that of Twist plasmid group (F = 27.214, 31.211;P < 0.05).

CONCLUSION:

As mice retinal neovascular growth, Twist may play important roles as a cell transition regulatory factor. Repressing Twist with RNAi, we can repress cell transition and inhibit retinal neovascular.

• Authors: Jun Li, Xiao-guang Dong, Ting Liu
• Journal: [Zhonghua yan ke za zhi] Chinese journal of ophthalmology (Zhonghua Yan Ke Za Zhi) Vol. 47 Issue 3 Pg. 217-22 (Mar 2011) ISSN: 0412-4081 [Print] China
• PMID: 21609622 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• RNA, Small Interfering
• Twist-Related Protein 1

Topics

• Animals
• Animals, Newborn
• Disease Models, Animal
• Gene Expression
• Hyperoxia
• Mice
• Mice, Inbred C57BL
• RNA, Small Interfering
• Retinal Neovascularization (pathology)
• Twist-Related Protein 1 (genetics)