Abstract | OBJECTIVE: To investigate the effect of Wnt/ beta-catenin signal pathway protein in the effect of Bushen Huoxue Granule (BHG) containing serum on the osteoblast, and to provide necessary experimental reliance for its action of mechanism in treatment of osteoporosis. METHODS: The osteoblast from cranial bones of neonates rat were isolated and cultured in vitro, which was divided into the blank control group and the BHG containing serum group. The alkaline phosphatase (ALP) activities of the osteoblast in each group were quantitatively detected and the ALP staining was performed six days later. The alizarin red staining was performed eighteen days later. At the same time, levels of Wnt/ beta-catenin signal pathway protein-- beta-catenin, low density lipoprotein correlated protein 5 (LRP 5), and T cell factor (TCF) of osteoblasts in each group were detected by ELISA. RESULTS: BHG containing serum could significantly increase the expression of ALP and promote the formation of mineralizing nodus in the osteoblasts. At the same time it also markedly up-regulated the expressions of p- catenin, LRP 5, and TCF in this process. CONCLUSION: BHG containing serum could markedly increase ossify activities and mineralization of osteoblast. This action was closely correlated with Wnt/p- catenin signal pathway. So it indicated that Wnt/ beta-catenin signal pathway played a very important role in the treatment of the osteoporosis by BHG.
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Authors | Ying-xing Xu, Cheng-liang Wu, Yan Wu |
Journal | Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine
(Zhongguo Zhong Xi Yi Jie He Za Zhi)
Vol. 31
Issue 4
Pg. 537-41
(Apr 2011)
ISSN: 1003-5370 [Print] China |
PMID | 21608229
(Publication Type: English Abstract, Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Drugs, Chinese Herbal
- Wnt Proteins
- beta Catenin
- bushen huoxue
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Topics |
- Animals
- Drugs, Chinese Herbal
(pharmacology)
- Female
- Osteoblasts
(drug effects, metabolism)
- Rats
- Rats, Sprague-Dawley
- Serum
(chemistry)
- Signal Transduction
(drug effects)
- Wnt Proteins
(metabolism)
- beta Catenin
(metabolism)
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