Monocyte deactivation correlates with injury severity score, but not with heme oxygenase-1 levels in trauma patients.

Abstract

Traumatic injury induces a local and systemic release of pro-inflammatory cytokines, acute phase proteins, hormones, and other inflammatory mediators. The excessive release of these mediators plays an important role in the pathogenesis of shock. In parallel to this pro-inflammatory response, there is a regulatory response characterized by the release of anti-inflammatory mediators, which is thought to represent the host's attempt to restore immunological equilibrium. Studies in septic patients have suggested the compensatory anti-inflammatory response may result in an "immunodeficient state" that leaves the host susceptible to further infectious insults. A key feature of the anti-inflammatory state in septic patients is a change in the responsiveness of monocytes that has been termed "monocyte deactivation." This is supported by data that link monocyte deactivation to increased mortality in septic patients. Monocytes with reduced HLA-DR expression have been described in trauma patients. We collected blood from 25 severely injured patients and evaluated peripheral blood mononuclear cells (PBMC) for HLA-DR expression and TNF-α response to LPS stimulation as markers of monocyte deactivation. Levels of intracellular HO-1 were determined in each patient, as HO-1 has been implicated in monocyte deactivation in patients with severe systemic inflammatory response syndrome (SIRS). HLA-DR expression correlated inversely with Injury Severity Scores and TNF-α response to LPS stimulation, but failed to correlate with HO-1 levels in these patients. HLA-DR expression was decreased in normal monocytes stimulated with patient plasma, but this treatment had no effect on HO-1 levels. These results suggest monocyte deactivation in trauma patients is unlikely to be mediated by HO-1.

• Authors: Sonlee D West, Carolyn Mold
• Journal: The Journal of surgical research (J Surg Res) Vol. 172 Issue 1 Pg. 5-10 (Jan 2012) ISSN: 1095-8673 [Electronic] United States
• PMID: 21601878 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier Inc. All rights reserved.

Chemical References

• Biomarkers
• HLA-DR Antigens
• Lipopolysaccharides
• Tumor Necrosis Factor-alpha
• Heme Oxygenase-1

Topics

• Adult
• Aged
• Biomarkers (metabolism)
• Case-Control Studies
• Female
• HLA-DR Antigens (metabolism)
• Heme Oxygenase-1 (blood)
• Humans
• Leukocytes, Mononuclear (drug effects, metabolism, pathology)
• Lipopolysaccharides (pharmacology)
• Male
• Middle Aged
• Plasma
• Trauma Severity Indices
• Tumor Necrosis Factor-alpha (metabolism)
• Wounds and Injuries (blood)