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Evaluation of the microscopic observation drug susceptibility assay for detection of Mycobacterium tuberculosis resistance to pyrazinamide.

Abstract
The microscopic observation drug susceptibility assay (MODS) was evaluated to determine susceptibility to pyrazinamide in Mycobacterium tuberculosis, and compared with the broth microdilution method (BMM), absolute concentration method (ACM), and pyrazinamidase (PZase) determination. We tested 34 M. tuberculosis clinical isolates (24 sensitive and eight resistant to pyrazinamide) and the control strains M. tuberculosis H37Rv (ATCC 27294) and Mycobacterium bovis AN5. The MODS, BMM, ACM and PZase determination provided results in average times of 6, 18, 28 and 7 days, respectively. All methods showed excellent sensitivity and specificity (p <0.05). Of the methods studied, the MODS proved to be faster, efficient, inexpensive, and easy to perform. However, additional studies evaluating the MODS in differentiating pyrazinamide-resistant and pyrazinamide-susceptible M. tuberculosis must be conducted with a larger number of clinical isolates.
AuthorsL D Ghiraldi, P A Z Campanerut, F L E Spositto, D N Sato, C Q F Leite, M Hirata, R D C Hirata, R F Cardoso
JournalClinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases (Clin Microbiol Infect) Vol. 17 Issue 12 Pg. 1792-7 (Dec 2011) ISSN: 1469-0691 [Electronic] England
PMID21595790 (Publication Type: Comparative Study, Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
Copyright© 2011 The Authors. Clinical Microbiology and Infection © 2011 European Society of Clinical Microbiology and Infectious Diseases.
Chemical References
  • Antitubercular Agents
  • Pyrazinamide
Topics
  • Antitubercular Agents (pharmacology)
  • Drug Resistance, Bacterial
  • Humans
  • Microbial Sensitivity Tests (methods)
  • Microscopy (methods)
  • Mycobacterium bovis (drug effects)
  • Mycobacterium tuberculosis (drug effects, isolation & purification)
  • Pyrazinamide (pharmacology)
  • Sensitivity and Specificity
  • Time Factors
  • Tuberculosis (microbiology)

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