Estramustine phosphate reversibly inhibits an early stage during adenovirus replication.

Abstract

Estramustine phosphate, an estradiol-mustard conjugate, was shown to reversibly inhibit a stage during the first hour of productive adenovirus 2 infection of HeLa cells. This drug, employed in the therapy of advanced prostatic cancer, specifically interacts with microtubule-associated proteins (MAPs) of the cytoskeleton. The results obtained under physiological conditions in vivo suggest a MAPs-interference with the microtubule-mediated vectorial migration of the virus inoculum to the nucleus. Virus attachment, uncoating kinetics and the appearance of established uncoating intermediates were not affected.

• Authors: E Everitt, H Ekstrand, B Boberg, B Hartley-Asp
• Journal: Archives of virology (Arch Virol) Vol. 111 Issue 1-2 Pg. 15-28 ( 1990) ISSN: 0304-8608 [Print] Austria
• PMID: 2158288 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Viral
• Microtubule-Associated Proteins
• Nitrogen Mustard Compounds
• Estramustine

Topics

• Adenoviridae Infections (microbiology)
• Adenoviruses, Human (drug effects, physiology)
• Antigens, Viral (biosynthesis)
• Capsid (biosynthesis)
• Centrifugation, Density Gradient
• Estramustine (pharmacology)
• HeLa Cells
• Humans
• Kinetics
• Microtubule-Associated Proteins (metabolism)
• Microtubules (drug effects, metabolism)
• Nitrogen Mustard Compounds (pharmacology)
• Virus Replication (drug effects)