Abstract | OBJECTIVE: METHODS: The expression and distribution of hnRNP A2/B1 were detected by immunohistochemistry and Western blot on 50 NSCLC samples from patients who underwent resection in Zhongshan Hospital. The hnRNP A2/B1 mRNA expression was tested by real-time PCR. Co-immunoprecipitation (co-IP) combined RT-PCR was used to investigate whether hnRNP A2/B1 could be bound with the mRNA of the above mentioned 5 DNA repair enzymes in human lung cancer cell line (HTB-182). Then immunohistochemistry and real-time PCR were used to detect the expression of MGMT in the same group of patients. RESULTS:
HnRNP A2/B1 protein and mRNA expressions were increased in the NSCLC tissues than that in the corresponding normal lung tissues. HnRNP A2/B1 was expressed predominantly in the nuclei of tumor cells. The positive rate and immunohistochemistry score of hnRNP A2/B1 in tumor tissue were significantly higher than that in normal tissue (P < 0.01). In stage III-IV NSCLC, hnRNP A2/B1 expression was higher than that in stage I-II. There was no significant differences of hnRNP A2/B1 expression among patients of different age, sex, histological type, and smoking history. The results of co-IP combined RT-PCR suggested that hnRNP A2/B1 is bound with MGMT mRNA, and MGMT expression is decreased in tumor tissue of NSCLC. CONCLUSIONS: The results of this study show that hnRNP A2/B1 protein and mRNA are highly expressed in NSCLC, and hnRNP A2/B1 is bound with MGMT mRNA, which indicate that it might be one of the mechanisms of hnRNP A2/B1 participating in the pathogenesis of NSCLC.
|
Authors | Yuan Shi, Ying Chen, Ying-yong Hou, Chun-hua Ji, Qin Hu, Yang Zhou, Jie-akesu Su, Yun-shan Tan |
Journal | Zhonghua zhong liu za zhi [Chinese journal of oncology]
(Zhonghua Zhong Liu Za Zhi)
Vol. 33
Issue 2
Pg. 110-4
(Feb 2011)
ISSN: 0253-3766 [Print] China |
PMID | 21575478
(Publication Type: English Abstract, Journal Article)
|
Chemical References |
- Heterogeneous-Nuclear Ribonucleoprotein Group A-B
- RNA, Messenger
- hnRNP A2
- 8-hydroxyguanine
- Guanine
- O-(6)-methylguanine
- DNA-Activated Protein Kinase
|
Topics |
- Blotting, Western
- Carcinoma, Non-Small-Cell Lung
(genetics)
- DNA-Activated Protein Kinase
(metabolism)
- Guanine
(analogs & derivatives)
- Heterogeneous-Nuclear Ribonucleoprotein Group A-B
(genetics, metabolism)
- Humans
- Immunohistochemistry
- Immunoprecipitation
- Lung
(chemistry)
- Lung Neoplasms
(genetics)
- RNA, Messenger
(analysis)
- Real-Time Polymerase Chain Reaction
- Reverse Transcriptase Polymerase Chain Reaction
|