Oxygenated perfluorocarbons protect the intestine from the ischemia/reperfusion injury in rabbits.

Abstract

OBJECTIVE:

To investigate whether intraluminal administration of oxygenated perfluorocarbons (PFCs) protects the enterocyte from acute ischemia-reperfusion (I/R) injury.

MATERIALS AND METHODS:

Twenty rabbits were divided in 4 groups: sham-operated controls (group A), acute I/R (group B), acute I/R plus infusion of oxygenated PFCs 30 minutes before ischemia (group C), and acute I/R plus infusion of oxygenated PFCs 30 minutes before reperfusion (group D). Serum creatine phosphokinase (CPK) and mucosal disaccharidase activity were examined. Intestinal biopsies were obtained for electron microscopy study.

RESULTS:

Group B CPK mean values are 3495.2 ± 157.35 and 4855 ± 350.21 U/L. Group C: 2674.6 ± 265.87 and 3231 ± 232.30. Group D: 2382.2 ± 102.90 and 3217.6 ± 185.61 at 120 and 180 minutes (P<.05). At 180 minutes, maltase and sucrose values were 33.63, 51.88, 8.45, and 19.91, and 17.99, 22.87, 6.62, and 14.24 µmol/min per g for groups A, B, C, and D, respectively (P<.05). Histopathology showed the least cellular deterioration in PFC groups.

CONCLUSION:

Oxygenated PFCs protect the enterocyte during bowel I/R.

• Authors: Achilleas Ntinas, Dionisios Vrochides, Stavros Iliadis, Georgios Papageorgiou, Athanasia Alvanou-Achparaki, Dimitrios Papadimitriou, Charalambos Spiridis, Thomas Gerasimidis
• Journal: Vascular and endovascular surgery (Vasc Endovascular Surg) Vol. 45 Issue 5 Pg. 426-32 (Jul 2011) ISSN: 1938-9116 [Electronic] United States
• PMID: 21571774 (Publication Type: Journal Article)
• Copyright: © The Author(s) 2011

Chemical References

• Fluorocarbons
• Protective Agents
• Creatine Kinase
• alpha-Glucosidases
• Sucrase
• Oxygen

Topics

• Analysis of Variance
• Animals
• Biopsy
• Creatine Kinase (blood)
• Cytoprotection
• Disease Models, Animal
• Enterocytes (drug effects, metabolism, pathology, ultrastructure)
• Fluorocarbons (pharmacology)
• Intestinal Mucosa (metabolism)
• Intestines (blood supply, drug effects, ultrastructure)
• Linear Models
• Microscopy, Electron
• Oxygen (pharmacology)
• Protective Agents (pharmacology)
• Rabbits
• Reperfusion Injury (prevention & control)
• Sucrase (metabolism)
• Time Factors
• alpha-Glucosidases (metabolism)