Abstract | AIMS: MAIN METHODS: Mouse J774A.1 cell line and mouse peritoneal macrophages were used. Gene expression and production of inflammatory mediators were determined by real-time PCR and Western blot. KEY FINDINGS: SIGNIFICANCE: Our findings imply that eupatilin suppresses inflammatory responses by the inhibition of NF-κB signaling pathway, and downstream inflammatory mediators in endotoxin-stimulated macrophages.
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Authors | Eun-Ju Choi, Soyoung Lee, Jeong-Ryong Chae, Hyun-Shik Lee, Chang-Duk Jun, Sang-Hyun Kim |
Journal | Life sciences
(Life Sci)
Vol. 88
Issue 25-26
Pg. 1121-6
(Jun 20 2011)
ISSN: 1879-0631 [Electronic] Netherlands |
PMID | 21565208
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2011 Elsevier Inc. All rights reserved. |
Chemical References |
- Cytokines
- Flavonoids
- Inflammation Mediators
- Lipopolysaccharides
- NF-kappa B
- lipopolysaccharide, E. coli O26-B6
- Nitric Oxide
- eupatilin
- Nitric Oxide Synthase Type II
- Nos2 protein, mouse
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Topics |
- Animals
- Artemisia
(chemistry)
- Blotting, Western
- Cell Culture Techniques
- Cell Line
- Cell Survival
(drug effects, immunology)
- Cytokines
(genetics, immunology)
- Dose-Response Relationship, Drug
- Flavonoids
(chemistry, isolation & purification, pharmacology)
- Gene Expression
(drug effects)
- Inflammation Mediators
(antagonists & inhibitors, immunology)
- Lipopolysaccharides
(pharmacology)
- Macrophages, Peritoneal
(drug effects, immunology)
- Mice
- Mice, Inbred BALB C
- Molecular Structure
- NF-kappa B
(antagonists & inhibitors, genetics, immunology)
- Nitric Oxide
(biosynthesis)
- Nitric Oxide Synthase Type II
(genetics)
- Reverse Transcriptase Polymerase Chain Reaction
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