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Eupatilin inhibits lipopolysaccharide-induced expression of inflammatory mediators in macrophages.

AbstractAIMS:
Eupatilin (5,7-dihydroxy-3,4,6-trimethoxyflavone) is a pharmacologically active ingredients in Stillen(TM), a drug for the gastric mucosal ulcers. Eupatilin has been known to possess anti-peptic, anti-cancer, and anti-allergy activity. In this report, we defined the effect of eupatilin on the endotoxin-induced inflammation in lipopolysaccharide (LPS)-stimulated macrophages.
MAIN METHODS:
Mouse J774A.1 cell line and mouse peritoneal macrophages were used. Gene expression and production of inflammatory mediators were determined by real-time PCR and Western blot.
KEY FINDINGS:
Eupatilin dose-dependently suppressed LPS-induced expression of inducible nitric oxide synthase (iNOS) and production of nitric oxide (NO). Eupatilin decreased LPS-induced expression of inflammatory mediators and pro-inflammatory cytokines such as cyclooxygenase-2, monocyte chemoattractant protein-1, tumor necrosis factor-α, interleukin (IL)-1β and IL-6. In addition, this suppression of inflammatory mediators was nuclear factor (NF)-κB dependent.
SIGNIFICANCE:
Our findings imply that eupatilin suppresses inflammatory responses by the inhibition of NF-κB signaling pathway, and downstream inflammatory mediators in endotoxin-stimulated macrophages.
AuthorsEun-Ju Choi, Soyoung Lee, Jeong-Ryong Chae, Hyun-Shik Lee, Chang-Duk Jun, Sang-Hyun Kim
JournalLife sciences (Life Sci) Vol. 88 Issue 25-26 Pg. 1121-6 (Jun 20 2011) ISSN: 1879-0631 [Electronic] Netherlands
PMID21565208 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2011 Elsevier Inc. All rights reserved.
Chemical References
  • Cytokines
  • Flavonoids
  • Inflammation Mediators
  • Lipopolysaccharides
  • NF-kappa B
  • lipopolysaccharide, E. coli O26-B6
  • Nitric Oxide
  • eupatilin
  • Nitric Oxide Synthase Type II
  • Nos2 protein, mouse
Topics
  • Animals
  • Artemisia (chemistry)
  • Blotting, Western
  • Cell Culture Techniques
  • Cell Line
  • Cell Survival (drug effects, immunology)
  • Cytokines (genetics, immunology)
  • Dose-Response Relationship, Drug
  • Flavonoids (chemistry, isolation & purification, pharmacology)
  • Gene Expression (drug effects)
  • Inflammation Mediators (antagonists & inhibitors, immunology)
  • Lipopolysaccharides (pharmacology)
  • Macrophages, Peritoneal (drug effects, immunology)
  • Mice
  • Mice, Inbred BALB C
  • Molecular Structure
  • NF-kappa B (antagonists & inhibitors, genetics, immunology)
  • Nitric Oxide (biosynthesis)
  • Nitric Oxide Synthase Type II (genetics)
  • Reverse Transcriptase Polymerase Chain Reaction

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