Primary study and anti-tumor mechanisms of analog from SC002.

Abstract

AIM:

To study the anti-tumor mechanisms of ascidiacea analog SC002.

METHODS:

After treated the tumor cells with ascidiacea analog SC002, the changes of a serials of tumor associated cell signal molecules, including VEGF (vascular endothelial growth factor), P53, P21 and Bcl-2 et al, were detected by Western blot; further more the expression of serum VEGF in tumor-bearing mice and the activity of Caspase-3, 8, 9 in tumor tissue were investigated by ELISA; at last.

RESULTS:

Ascidiacea analog SC002 can obviously induce the expression of P53, while decreasing the expression of Bcl-2 and VEGF, but have on effect on p21 in tumor tissue; ELISA shown that SC002 can also reduce the concentration of serum VEGF in tumor bearing mice, but did not affected the activity of Caspase-3, 8 and 9.

CONCLUSION:

SC002 have the anti-tumor function through promoting the expression of tumor suppressor gene p53, supress the expression of Bcl-2 and VEGF. This founding will provided an important base theory for it's further use as a novel anti-tumor drugs.

• Authors: Shu-ping Chi, Rong Gao, Chen Wang, Li-yue You, Hai-min Lei, Yun Cheng, Jin Li
• Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi) Vol. 27 Issue 2 Pg. 212-4 (Feb 2011) ISSN: 1007-8738 [Print] China
• PMID: 21560442 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• Cephalosporins
• Proto-Oncogene Proteins c-bcl-2
• Tumor Suppressor Protein p53
• Vascular Endothelial Growth Factor A
• Caspases
• SC002

Topics

• Animals
• Antineoplastic Agents (pharmacology)
• Caspases (metabolism)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Cephalosporins (pharmacology)
• Female
• Hep G2 Cells
• Humans
• Mice
• Mice, Inbred BALB C
• Proto-Oncogene Proteins c-bcl-2 (metabolism)
• Signal Transduction (drug effects)
• Transplantation, Heterologous
• Tumor Suppressor Protein p53 (metabolism)
• Vascular Endothelial Growth Factor A (metabolism)