Downregulation of caspase-10 predicting poor survival after resection of stage II colorectal cancer.

Abstract

PURPOSE:

The aim of this study was to evaluate the prevalence and clinical significance of caspase-10 mRNA expression in stage II colorectal cancer.

METHODS:

Quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) was used to analyze caspase-10 expression in cancer tissue and corresponding normal mucosa from 120 patients with stage II colorectal cancer. Variables were analyzed by Chi-square test or Fisher's exact test. Survival was evaluated with method of Kaplan-Meier. Multivariate analysis was performed with Cox's proportional hazards model.

RESULTS:

The expression of caspase-10 mRNA was found to be downregulated in cancer tissue compared to normal mucosa (P = 0.001). Poorly differentiated cancer showed lower mRNA expression than cancer with greater differentiation (P = 0.031). Univariate survival curves, estimated using the method of Kaplan-Meier, defined a significant association between caspase-10 expression and both overall survival (P = 0.012) and disease-free survival (P = 0.021). A multivariate analysis, performed by Cox's proportional hazards regression model, confirmed that a low caspase-10 expression was the only significant factor to predict poor prognosis in patients with stage II colorectal cancer.

CONCLUSION:

Our data indicate that caspase-10 expression, measured by quantitative real-time RT-PCR, is a possible prognostic factor in patients with stage II colorectal cancer.

• Authors: Xiao-Gang Shen, Cun Wang, Yuan Li, Bin Zhou, Bin Xu, Lie Yang, Zong-Guang Zhou, Xiao-Feng Sun
• Journal: International journal of colorectal disease (Int J Colorectal Dis) Vol. 26 Issue 12 Pg. 1519-24 (Dec 2011) ISSN: 1432-1262 [Electronic] Germany
• PMID: 21559821 (Publication Type: Journal Article)

Chemical References

• RNA, Messenger
• Caspase 10
• CASP10 protein, human

Topics

• Adult
• Aged
• Caspase 10 (genetics, metabolism)
• Colorectal Neoplasms (enzymology, genetics, pathology, surgery)
• Disease-Free Survival
• Down-Regulation (genetics)
• Female
• Gene Expression Regulation, Enzymologic
• Gene Expression Regulation, Neoplastic
• Humans
• Intestinal Mucosa (enzymology, pathology)
• Male
• Middle Aged
• Multivariate Analysis
• Neoplasm Staging
• Prognosis
• RNA, Messenger (genetics, metabolism)
• Regression Analysis
• Software
• Up-Regulation (genetics)