Abstract |
Solar ultraviolet (UV) radiation causes skin damage including increases in skin thickness, edema, and flush. In this study, we examined the effects of two main flavonoids ( wogonin and baicalein) isolated from the roots of Scutellaria baicalensis, a traditional remedy for allergic inflammatory diseases long used in China and Japan, on acute UVB irradiation-induced skin damage in hairless mice. Baicalein and wogonin (10 or 50 mg/kg) were administered orally twice daily for 14 consecutive days. The UVB irradiation was performed at a dose of 200 mJ/cm(2) on days 7 and 8 of the treatment with the two main flavonoids. Baicalein, and wogonin prevented the increases in skin thickness and the levels of matrix metalloproteinase (MMP)-9, and vascular endothelial growth factor ( VEGF) induced by the irradiation. Wogonin reduced the levels of cyclooxygenase (COX)-2 and hypoxia inducible factor (HIF)-1α in UVB-treated HaCaT cells. These findings suggest that wogonin inhibits irradiation-induced skin damage by suppressing increases in the levels of MMP-9, and VEGF through the inhibition of COX-2 and HIF-1α expression. Baicalein inhibited COX-2 and NF-κB/p65 expression, but stimulated HIF-1α expression. Therefore, its inhibitory action is likely due to the expression of MMP-9 and VEGF through the suppression of COX-2 and NF-κB/p65 expression. Furthermore, the inhibitory effects of baicalein on UVB-irradiated hyperplasia of skin epidermis may be due to the stimulation of HIF-1α expression.
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Authors | Yoshiyuki Kimura, Maho Sumiyoshi |
Journal | European journal of pharmacology
(Eur J Pharmacol)
Vol. 661
Issue 1-3
Pg. 124-32
(Jul 01 2011)
ISSN: 1879-0712 [Electronic] Netherlands |
PMID | 21549115
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2011 Elsevier B.V. All rights reserved. |
Chemical References |
- Flavanones
- Hif1a protein, mouse
- Hypoxia-Inducible Factor 1, alpha Subunit
- Rela protein, mouse
- Transcription Factor RelA
- Vascular Endothelial Growth Factor A
- baicalein
- Cyclooxygenase 2
- Matrix Metalloproteinase 2
- Matrix Metalloproteinase 9
- wogonin
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Topics |
- Animals
- Blood Vessels
(drug effects, pathology)
- Cell Line
- Cell Nucleus
(drug effects, metabolism)
- Cyclooxygenase 2
(metabolism)
- Ear
(pathology)
- Epidermis
(drug effects, pathology)
- Extracellular Matrix
(drug effects, metabolism)
- Flavanones
(isolation & purification, pharmacology)
- Gene Expression Regulation
(drug effects)
- Humans
- Hypoxia-Inducible Factor 1, alpha Subunit
(metabolism)
- Male
- Matrix Metalloproteinase 2
(metabolism)
- Matrix Metalloproteinase 9
(metabolism)
- Mice
- Mice, Hairless
- Plant Roots
(chemistry)
- Scutellaria baicalensis
(chemistry)
- Skin
(drug effects, metabolism, pathology, radiation effects)
- Transcription Factor RelA
(metabolism)
- Ultraviolet Rays
(adverse effects)
- Vascular Endothelial Growth Factor A
(metabolism)
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