Abstract |
Intragastric (i.g.) immunization with recombinant chimeric proteins constructed from the saliva-binding region (SBR) of Streptococcus mutans surface antigen AgI/II and the A2/B subunits of enterobacterial heat-labile enterotoxins has been successfully used to induce salivary and circulating antibodies against S. mutans that have protective potential against dental caries. To investigate the mode of action of these vaccine constructs, mice were immunized i.g. with chimeric proteins constructed from SBR and cholera toxin (CT) or the type II enterotoxins of Escherichia coli, LT-IIa and LT-IIb. Antigen-presenting cells (APC) in Peyer's patches (PP) and mesenteric lymph nodes (MLN) were characterized by flow cytometry. Compared with immunization with SBR alone, chimeric proteins SBR-LTIIaA2/B and SBR-LTIIbA2/B increased the number of B cells and macrophages in PP and diminished B cell numbers in MLN, whereas SBR-CTA2/B diminished the numbers of B cells and macrophages in PP and MLN. Immunization with all three chimeric proteins led to upregulation of MHC class II molecules and co-stimulatory receptors CD40, CD80, and CD86 especially on dendritic cells in PP and also on APC in MLN. The results provide a molecular basis for the enhanced immune responses induced by chimeric proteins compared with uncoupled antigen, and for differential responses to chimeric proteins based on CT or type II enterotoxins.
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Authors | W Zhao, Z Zhao, M W Russell |
Journal | Molecular oral microbiology
(Mol Oral Microbiol)
Vol. 26
Issue 3
Pg. 200-9
(Jun 2011)
ISSN: 2041-1014 [Electronic] Denmark |
PMID | 21545697
(Publication Type: Comparative Study, Journal Article, Research Support, N.I.H., Extramural)
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Copyright | © 2011 John Wiley & Sons A/S. |
Chemical References |
- Antigens, Bacterial
- B7-1 Antigen
- B7-2 Antigen
- Bacterial Proteins
- Bacterial Toxins
- CD40 Antigens
- Cd86 protein, mouse
- Enterotoxins
- Escherichia coli Proteins
- Histocompatibility Antigens Class II
- Recombinant Fusion Proteins
- Salivary Proteins and Peptides
- Streptococcal Vaccines
- Vaccines, Synthetic
- Cholera Toxin
- heat-labile enterotoxin, E coli
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Topics |
- Animals
- Antigen-Presenting Cells
(immunology)
- Antigens, Bacterial
(immunology)
- B-Lymphocytes
(immunology)
- B7-1 Antigen
(immunology)
- B7-2 Antigen
(immunology)
- Bacterial Proteins
(immunology)
- Bacterial Toxins
(immunology)
- CD40 Antigens
(immunology)
- Cholera Toxin
(immunology)
- Dendritic Cells
(immunology)
- Enterotoxins
(immunology)
- Escherichia coli Proteins
(immunology)
- Female
- Histocompatibility Antigens Class II
(immunology)
- Immunization
- Intestinal Mucosa
(immunology)
- Lymph Nodes
(immunology)
- Macrophages
(immunology)
- Mesentery
(immunology)
- Mice
- Mice, Inbred BALB C
- Peyer's Patches
(immunology)
- Recombinant Fusion Proteins
(administration & dosage, immunology)
- Salivary Proteins and Peptides
(immunology)
- Stomach
- Streptococcal Vaccines
(administration & dosage, immunology)
- Streptococcus mutans
(immunology)
- Up-Regulation
- Vaccines, Synthetic
(administration & dosage, immunology)
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