Evaluation of bacteriochlorophyll-reconstituted low-density lipoprotein nanoparticles for photodynamic therapy efficacy in vivo.

Abstract

AIM:

To evaluate the novel nanoparticle reconstituted bacteriochlorin e6 bisoleate low-density lipoprotein (r-Bchl-BOA-LDL) for its efficacy as a photodynamic therapy agent delivery system in xenografts of human hepatoblastoma G2 (HepG2) tumors.

MATERIALS & METHODS:

Bchl-BOA was encapsulated in the nanoparticle low-density lipoprotein (LDL), a native particle whose receptor's overexpression is a cancer signature for a number of neoplasms. Evaluation of r-Bchl-BOA-LDL as a potential photosensitizer was performed using a tumor response and foot response assay.

RESULTS & DISCUSSION:

When compared with controls, tumor regrowth was significantly delayed at injected murine doses of 2 µmole/kg r-Bchl-BOA-LDL after illumination at fluences of 125, 150 or 175 J/cm(2). Foot response assays showed that although normal tissue toxicity accompanied the higher fluences it was significantly reduced at the lowest fluence tested.

CONCLUSION:

This research demonstrates that r-Bchl-BOA-LDL is an effective photosensitizer and a promising candidate for further investigation.

• Authors: Diane E Marotta, Weiguo Cao, E Paul Wileyto, Hui Li, Ian Corbin, Elizabeth Rickter, Jerry D Glickson, Britton Chance, Gang Zheng, Theresa M Busch
• Journal: Nanomedicine (London, England) (Nanomedicine (Lond)) Vol. 6 Issue 3 Pg. 475-87 (Apr 2011) ISSN: 1748-6963 [Electronic] England
• PMID: 21542686 (Publication Type: Evaluation Study, Journal Article)

Chemical References

• Photosensitizing Agents

Topics

• Animals
• Cell Line, Tumor
• Drug Delivery Systems
• Humans
• Mice
• Mice, Nude
• Nanoparticles (administration & dosage, chemistry)
• Neoplasms (drug therapy)
• Photochemotherapy (methods)
• Photosensitizing Agents (administration & dosage)
• Xenograft Model Antitumor Assays