Protection of cattle and swine against foot-and-mouth disease, using biosynthetic peptide vaccines.

Abstract

A single dose of foot-and-mouth disease (FMD) virus protein 1 (VP1) peptide, expressed in Escherichia coli as a fusion protein with 190 amino acids (AA) of the LE' protein of the tryptophan operon of E coli, elicited an immune response in steers sufficient to withstand the challenge of exposure to animals with acute FMD. The 58-micrograms dose of viral peptide, composed of a segment of the VP1 from the A12 strain (A12) of FMD virus (FMDV; A12-32dimer) in a tandem repeat configuration of AA137 through 168 and emulsified with oil adjuvant, elicited a serologic response in cattle equivalent to that obtained using conventional whole virus vaccines. Two groups of swine were vaccinated, 1 with the A12-32dimer as used in cattle and 1 with AA131 through 157 from VP1 of the A24 strain (A24) of FMDV (A24-peptide), expressed in the same system as A12-32dimer, but as a single copy per molecule. In swine, the 58-micrograms dose of the A12-32dimer repeated at 28 days was an effective immunogen; all swine were protected against A12 and, in addition, the vaccine protected 50% of the swine against A24. The 29-micrograms dose of A24-peptide, administered according to the same schedule, elicited protection against A24 in 50% of the vaccinates and, in addition, protected 25% of those vaccinates against A12. The serologic response elicited by A12-32dimer against A24 virus was considerably greater than the response elicited by A24-peptide against A12 virus. The evidence of multiple immunogenic epitopes between AA131 and AA168 was evaluated.

• Authors: D O Morgan, D M Moore
• Journal: American journal of veterinary research (Am J Vet Res) Vol. 51 Issue 1 Pg. 40-5 (Jan 1990) ISSN: 0002-9645 [Print] United States
• PMID: 2154148 (Publication Type: Journal Article)

Chemical References

• Recombinant Fusion Proteins
• Vaccines
• Vaccines, Synthetic
• Viral Vaccines

Topics

• Amino Acid Sequence
• Animals
• Aphthovirus (immunology)
• Cattle
• Cattle Diseases (immunology, prevention & control)
• Female
• Foot-and-Mouth Disease (immunology, prevention & control)
• Genetic Engineering (veterinary)
• Male
• Molecular Sequence Data
• Recombinant Fusion Proteins (genetics)
• Swine
• Swine Diseases (immunology, prevention & control)
• Vaccination (veterinary)
• Vaccines (immunology)
• Vaccines, Synthetic (analysis, immunology)
• Viral Vaccines (analysis, immunology)