Role of nucleotide pyrophosphatase in enflurane-induced reduction of UDP-glucuronic acid concentration in mouse liver.

Abstract

Because hepatic UDP-glucuronic acid levels decrease upon exposure to volatile anesthetics, the present study was designed to determine the mechanism by which enflurane decreases UDP-glucuronic acid in mice by measuring the concentrations of intermediates and the activities of enzymes in the UDP-glucuronic acid pathway. UDP-glucuronic acid concentrations were decreased by 40% in both male and female mice after 10 min of enflurane-induced narcosis. Concentration of UDP-glucose and the activities of diethylstilbestrol UDP-glucuronosyltransferase and UDP-glucose dehydrogenase were not affected by enflurane treatment. In contrast, nucleotide pyrophophatase activity was increased approximately 50% in both sexes. Thus, the decrease in hepatic UDP-glucuronic acid upon exposure of mice to enflurane is probably due to increased degradation by nucleotide pyrophosphatase.

• Authors: J B Watkins 3rd, M A Pierce
• Journal: Toxicology and applied pharmacology (Toxicol Appl Pharmacol) Vol. 102 Issue 2 Pg. 378-83 (Feb 1990) ISSN: 0041-008X [Print] United States
• PMID: 2154067 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Uridine Diphosphate Sugars
• Uridine Diphosphate Glucuronic Acid
• Enflurane
• Pyrophosphatases
• nucleotide pyrophosphatase

Topics

• Animals
• Enflurane (pharmacology)
• Female
• Male
• Mice
• Microsomes, Liver (enzymology)
• Pyrophosphatases (metabolism)
• Uridine Diphosphate Glucuronic Acid (metabolism)
• Uridine Diphosphate Sugars (metabolism)