Abstract | BACKGROUND: The effectiveness of the URIT-2900 Hematology Analyzer for screening of hemoglobinopathies commonly found in Southeast Asian populations was examined. METHODS: Appropriate cut-off values of MCV and MCH for screening of α(0) and β thalassemias were derived from the receiver operator characteristic curve conducted initially on 279 subjects with various thalassemia genotypes. Validation was performed additionally in a cohort of another unrelated 313 subjects. RESULTS: The best cut off values of MCV and MCH were found to be 78fL and 27pg, respectively. Using these cut off values in combination with the dichlorophenolindophenol test in screening of α(0) thalassemia, β thalassemia and Hb E in a cohort study revealed 100% sensitivity, 79.6% specificity, 80.0% positive predictive value and 100% negative predictive value. CONCLUSION:
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Authors | Rossarin Karnpean, Anupong Pansuwan, Goonnapa Fucharoen, Supan Fucharoen |
Journal | Clinical biochemistry
(Clin Biochem)
Vol. 44
Issue 10-11
Pg. 889-93
(Jul 2011)
ISSN: 1873-2933 [Electronic] United States |
PMID | 21539820
(Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2011 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved. |
Chemical References |
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Topics |
- Asia, Southeastern
- Automation
- Cellular Senescence
- Cohort Studies
- Erythrocyte Indices
- Genotype
- Hematology
(instrumentation)
- Hemoglobins
(metabolism)
- Humans
- Mass Screening
- ROC Curve
- Reproducibility of Results
- Thalassemia
(blood, diagnosis, genetics, pathology)
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