Fenthion and terbufos induce DNA damage, the expression of tumor-related genes, and apoptosis in HEPG2 cells.

Abstract

This study investigates the effects of fenthion and terbufos, two organophosphorous pesticides, on DNA damage, tumor-related gene expression, and apoptosis in HepG2 cells. We found that exposure to concentrations ranging from 50 to 200 μM of fenthion and terbufos for 2 hr caused significant death in HepG2 cells. Both compounds induced DNA damage in a concentration-dependent manner as measured using the alkaline comet assay. Tumor-related genes (jun, myc, and fos) and apoptosis-related genes (socs3, tnfaip3, ppp1r15a, and nr4a1) were up-regulated by both compounds. Finally, both compounds induced apoptosis. The results demonstrate that both terbufos and fenthion induce DNA damage and should be considered potentially hazardous to humans.

• Authors: Jong-C Wu, You-Cheng Hseu, Jui-Shan Tsai, Lei-Chin Chen, Soi M Chye, Chin-H Chen, Ssu Ching Chen
• Journal: Environmental and molecular mutagenesis (Environ Mol Mutagen) Vol. 52 Issue 7 Pg. 529-37 (Aug 2011) ISSN: 1098-2280 [Electronic] United States
• PMID: 21538558 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Wiley-Liss, Inc.

Chemical References

• Organothiophosphorus Compounds
• Pesticides
• Fenthion
• terbufos

Topics

• Apoptosis (drug effects)
• Cell Survival (drug effects)
• Comet Assay
• DNA Damage (drug effects)
• Fenthion (toxicity)
• Gene Expression Regulation, Neoplastic (drug effects)
• Hep G2 Cells
• Humans
• Organothiophosphorus Compounds (toxicity)
• Pesticides (toxicity)