Clinical significance of pyrimidine nucleoside phosphorylase in colorectal carcinoma.

Abstract

The expression of pyrimidine nucleoside phosphorylase (PyNPase) mRNA in tumor (T) and normal (N) biopsy specimens obtained from 41 cases of colorectal carcinoma was examined by the reverse transcriptase/polymerase chain reaction (RT-PCR). The higher T/N ratio of the expression of PyNPase mRNA correlated significantly with the presence of lymph vessel invasion (p=0.039), the positive lymph node metastasis (p=0.014) and the advanced stage of the disease (p=0.014). There was a significant con-elation between the results determined by enzyme activity and those determined by RT-PCR (p=0.005). The findings suggested that the determination of PyNPase mRNA by RT-PCR may give useful information on tumor aggressiveness of colorectal carcinoma and this method can be used instead of enzyme activity.

• Authors: K Mimori, M Mori, T Shiraishi, M Haraguchi, H Ueo, T Akiyoshi
• Journal: International journal of oncology (Int J Oncol) Vol. 10 Issue 3 Pg. 493-6 (Mar 1997) ISSN: 1019-6439 [Print] Greece
• PMID: 21533402 (Publication Type: Journal Article)