Abstract | OBJECTIVE: METHODS: MCF-7 cells were stably transfected with PGRMC1 expression plasmid (MCF-7/PGRMC1-3HA [WT-12]). To test the effects of E2 and progestogens on cell proliferation, MCF-7 and WT-12 cells were stimulated with different concentrations of E2 (10 and 10 M) alone and in combination with progesterone and medroxyprogesterone acetate (each 10 M). RESULTS: E2 elicited a concentration-dependent proliferative effect on both cell lines, which was much more pronounced in WT-12 cells (50% vs 200%). This effect could be completely abrogated by the addition of the E2 antagonist fulvestrant. Addition of progesterone had no influence on the E2-induced effect, whereas medroxy- progesterone acetate enhanced the E2-induced effect at a low E2 concentration, which was, again, more pronounced in the WT-12 cells. The figures were between 20% and 40% in MCF-7 and between 60% and 250% in WT-12 cells. CONCLUSIONS:
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Authors | Hans Neubauer, Yang Yang, Harald Seeger, Tanja Fehm, Michael A Cahill, Xiaowen Tong, Xiangyan Ruan, Alfred O Mueck |
Journal | Menopause (New York, N.Y.)
(Menopause)
Vol. 18
Issue 8
Pg. 845-50
(Aug 2011)
ISSN: 1530-0374 [Electronic] United States |
PMID | 21532513
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Contraceptive Agents, Female
- Membrane Proteins
- PGRMC1 protein, human
- Receptors, Progesterone
- Fulvestrant
- Estradiol
- Medroxyprogesterone Acetate
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Topics |
- Breast Neoplasms
(metabolism, pathology)
- Cell Division
(drug effects)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Contraceptive Agents, Female
(pharmacology)
- Dose-Response Relationship, Drug
- Estradiol
(analogs & derivatives, pharmacology)
- Female
- Fulvestrant
- Humans
- Medroxyprogesterone Acetate
(pharmacology)
- Membrane Proteins
(metabolism)
- Receptors, Progesterone
(metabolism)
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