Abstract | BACKGROUND: METHODS: ST- silk protein membranes (thickness, 100 μm; pore size, <100 nm) were loaded with log-scale colistin dilutions (0.027 to 270 mg/ml) and tested in a modified microbroth dilution assay against Pseudomonas aeruginosa (American Type Culture Collection 27853). A rat burn infection model was used to demonstrate the antimicrobial activity of ST- silk membranes loaded with 270 mg/ml colistin. Finally, a porcine wound infection model was used to study dose response (2.7, 27, and 270 mg/ml colistin loading concentration) in a time-dependent manner (0, 2, 4, and 6 days). RESULTS: The in vitro study demonstrated a concentration-dependent antimicrobial effect against P. aeruginosa, with complete elimination at the highest loading concentrations (2.7, 27, and 270 mg/ml). All colistin membranes demonstrated lower colony-forming unit counts compared with the corresponding phosphate-buffered saline or carrier controls. The rat burn infection model demonstrated a colony-forming unit reduction of greater than 3 log-scales for the colistin-loaded ST- silk membranes after 3 days. On average, the wounds' colony-forming unit quantity remained at greater than 1000 during the entire follow-up of 6 days, apart from three wounds where complete bacterial clearance was observed. CONCLUSION:
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Authors | Lars Steinstraesser, Galina Trust, Andrea Rittig, Tobias Hirsch, Marco R Kesting, Hans-Ulrich Steinau, Frank Jacobsen |
Journal | Plastic and reconstructive surgery
(Plast Reconstr Surg)
Vol. 127
Issue 5
Pg. 1838-1846
(May 2011)
ISSN: 1529-4242 [Electronic] United States |
PMID | 21532413
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Anti-Bacterial Agents
- Coated Materials, Biocompatible
- Membranes, Artificial
- Silk
- Colistin
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Topics |
- Animals
- Anti-Bacterial Agents
(pharmacology)
- Bandages
- Burns
(therapy)
- Coated Materials, Biocompatible
- Colistin
(pharmacology)
- Disease Models, Animal
- Membranes, Artificial
- Pseudomonas Infections
(microbiology, therapy)
- Pseudomonas aeruginosa
(isolation & purification)
- Rats
- Rats, Sprague-Dawley
- Silk
- Stem Cells
(drug effects)
- Treatment Outcome
- Wound Infection
(microbiology, therapy)
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