[Expression of NKG2D and NKG2A with their ligands MHC-I A/B and HLA-E in acute leukemia patients and its significance].

Abstract

This study was aimed to explore the difference of NK cell receptor NKG2D and NKG2A expression on NK cells and CD3(+) T cells and their ligand MHC-I A/B (major histocompatibility complex class I-related chains A/B) and HLA-E expression in leukemia cells, as well as its immunological significance. Flow cytometry was used to detect the killing rate of NK92 cells to 8 leukemia cell lines, and the expression of NKG2D and NKG2A on NK cells and CD3(+) T cells as well as their ligand MHC-I A/B and HLA-E expression on leukemia cells. The results indicated that the NK92 showed different killing activity to different leukemia cell lines. The positive expression rate of NKG2D and NKG2A on NK cells and CD3(+) T cells in ALL patients was no significantly different from that in AML patients (p > 0.05), but positive expression rate of MHC-I A/B and HLA-E in ALL patients was obviously higher than that in AML patients (p < 0.05). It is concluded that there is difference of immune cell function between ALL and AML patients, this difference may be associated with the expression difference of NKG2D and NKG2A ligands on leukemia cells while does not associated with the killing and inhibiting receptors expressed on NK cells and CD3(+) T cells.

• Authors: Shu-Jing Ge, Lian-Ning Duan, Yuan Luo, Ta-Lin Suo, Cheng-Rong Lu, Jie Tang
• Journal: Zhongguo shi yan xue ye xue za zhi (Zhongguo Shi Yan Xue Ye Xue Za Zhi) Vol. 19 Issue 2 Pg. 312-6 (Apr 2011) ISSN: 1009-2137 [Print] China
• PMID: 21518478 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• HLA-E antigen
• Histocompatibility Antigens Class I
• KLRC1 protein, human
• KLRK1 protein, human
• MHC class I-related chain A
• MICB antigen
• NK Cell Lectin-Like Receptor Subfamily C
• NK Cell Lectin-Like Receptor Subfamily K

Topics

• Adolescent
• Adult
• Aged
• Aged, 80 and over
• Child
• Child, Preschool
• Female
• Histocompatibility Antigens Class I (genetics, metabolism)
• Humans
• Infant
• Leukemia, Myeloid, Acute (genetics, metabolism)
• Male
• Middle Aged
• NK Cell Lectin-Like Receptor Subfamily C (genetics, metabolism)
• NK Cell Lectin-Like Receptor Subfamily K (genetics, metabolism)
• Precursor Cell Lymphoblastic Leukemia-Lymphoma (genetics, metabolism)
• Young Adult