[Investigation of peroxide modification of low- and very low-density lipoproteins in patients with coronary heart disease by the method of fluorescent spectroscopy].

In the total fraction of low and very low density lipoproteins (LDL+VLDL) isolated from serum by precipitation in the presence of heparin-Mn the copper-induced lipid peroxidation was accompanied by accumulation of LPO products, a decrease ANS fluorescence intensity (F(ANS)) and an increase in probe--cation DSP-6, a fluorescence intensity decrease of intrinsic in the ultraviolet area (F(uv)) and an increase in the visible area (F(vis)). The degree of lipoprotein modification was estimated by calculating the F(vis)/F(uv) and F(DSP)-6/F(ANS) ratio. Strong positive correlation was found between these ratios and concentration of thiobarbituric acid-reactive substances (TBARS) of LDL+VLDL samples isolated from sera of 49 donors and incubated at 37 degrees C in the presence of 50 M CuSO4 during 0, 3 and 24 hr (F(vis)/F(uv) (r = 0.75; p < 0.001) and F(DSP-6)/F(ANS) (r = 0.73; p < 0.001)). Very strong positive correlation was also found between both fluorescent parameters F(vis)/F(uv) and F(DSP-6)/F(ANS) (r = 0.95, p < 0.001). Changes in the values of F(vis)/ F(uv), F(DSP-6)/F(ANS), concentration of TBARS in 75 patients with documented coronary heart disease (CHD) and 49 apparently healthy donors were studied. No significant differences of these parameters in LDL+VLDL of patients with CHD and donors were found.
AuthorsR K Aĭdyraliev, O A Igemberdieva, M Kh Dadabaev, T M Murataliev, K A Aĭtbaev, A A Aldashev
JournalBiomedit︠s︡inskai︠a︡ khimii︠a︡ (Biomed Khim) 2011 Jan-Feb Vol. 57 Issue 1 Pg. 127-32 ISSN: 2310-6972 [Print] Russia (Federation)
PMID21516784 (Publication Type: English Abstract, Journal Article)
Chemical References
  • Fibrinolytic Agents
  • Lipoproteins, LDL
  • Lipoproteins, VLDL
  • Thiobarbituric Acid Reactive Substances
  • Manganese
  • Copper
  • Heparin
  • Copper (pharmacology)
  • Coronary Disease (blood)
  • Female
  • Fibrinolytic Agents (pharmacology)
  • Heparin (pharmacology)
  • Humans
  • Lipid Peroxidation (drug effects)
  • Lipoproteins, LDL (blood, chemistry)
  • Lipoproteins, VLDL (blood, chemistry)
  • Male
  • Manganese (pharmacology)
  • Spectrometry, Fluorescence
  • Thiobarbituric Acid Reactive Substances (analysis, chemistry)

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