Abstract |
Validation of new therapeutic targets calls for the advance in innovative assays that probe both spatial and temporal relationships in signaling networks. Cell death assays have already found a widespread use in pharmacological profiling of anticancer drugs. Such assays are, however, predominantly restricted to end point DEAD/LIVE parameter that provides only a snapshot of inherently stochastic process such as tumor cell death. Development of new methods that can offer kinetic real-time analysis would be highly advantageous for the pharmacological screening and predictive toxicology. In the present work we outline innovative protocols for the real-time analysis of tumor cell death, based on propidium iodide (PI) and SYTOX Green probes. These can be readily adapted to both flow cytometry and time-lapse fluorescence imaging. Considering vast time savings and kinetic data acquisition such assays have the potential to be applied in a number of areas including accelerated anticancer drug discovery and high-throughput screening routines.
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Authors | Donald Wlodkowic, Shannon Faley, Zbigniew Darzynkiewicz, Jonathan M Cooper |
Journal | Methods in molecular biology (Clifton, N.J.)
(Methods Mol Biol)
Vol. 731
Pg. 285-91
( 2011)
ISSN: 1940-6029 [Electronic] United States |
PMID | 21516415
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Topics |
- Animals
- Cell Death
(drug effects)
- Cell Line, Tumor
- Drug Screening Assays, Antitumor
(instrumentation, methods)
- Flow Cytometry
- Humans
- Molecular Imaging
- Time Factors
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