Profiling of a few immune responsive genes expressed in postlarvae of Fenneropenaeus indicus challenged with Vibrio harveyi D3.

Abstract

We identified 38 genes and eight hypothetical proteins by sequencing of 1200 clones from a Vibrio harveyi challenged Fenneropenaeus indicus subtracted cDNA library. Based on physiological roles and functions these genes were categorized into 10 groups with ∼29% of the sequences having no matches in the databases. Immune related transcripts in the library were carboxypeptidase B, ferritin, translationally controlled tumor proteins (TCTP), hemocyanin, chitinase and serine carboxy peptidase. Remarkably, qPCR results imply 4.15, 3.45 and 1.86-fold rises in expression of ferritin, TCTP and hemocyanin transcripts respectively. Additionally, minor upregulation of other immune relevant genes lectin, penaeidin, crustin, MnSOD was observed in the challenged postlarvae.

• Authors: S Nayak, K M Ajay, N Ramaiah, Ram M Meena, R A Sreepada
• Journal: Journal of invertebrate pathology (J Invertebr Pathol) Vol. 107 Issue 2 Pg. 168-72 (Jun 2011) ISSN: 1096-0805 [Electronic] United States
• PMID: 21515280 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier Inc. All rights reserved.

Topics

• Animals
• Aquaculture
• DNA Virus Infections (immunology)
• Gene Expression
• Gene Expression Profiling
• Gene Expression Regulation
• Larva (genetics, immunology, microbiology)
• Penaeidae (genetics, immunology, microbiology)
• Vibrio (immunology)
• Vibrio Infections (immunology)