Rapid HCP5 single-nucleotide polymorphism genotyping: a simple allele-specific PCR method for prediction of hypersensitivity reaction to Abacavir.

Abstract

BACKGROUND:

The most important factor limiting the success of an antiretroviral therapy is toxicity. The HLA-B*5701 allele is predictive of hypersensitivity reaction to Abacavir, and this gene is in a perfect linkage disequilibrium with the rs2395029 SNP present in the HCP5 gene.

METHODS:

Genomic DNA was extracted from blood obtained from 201 unrelated healthy Argentinean volunteers. The DNA was subjected to an allele-specific PCR method. Sequencing was performed to validate the test results.

RESULTS:

We were successful to amplify specific fragment of interest from the DNA samples. The method is easy, specific and reproducible.

CONCLUSIONS:

The application of this methodology is a rapid and simple method to detect the HCP5 polymorphism (rs2395029) previous to administration of Abacavir in patients with HIV infection.

• Authors: Cristian A Galván, Osvaldo C Elbarcha, Eduardo J Fernández, Dante M Beltramo, Néstor Walter Soria
• Journal: Clinica chimica acta; international journal of clinical chemistry (Clin Chim Acta) Vol. 412 Issue 15-16 Pg. 1382-4 (Jul 15 2011) ISSN: 1873-3492 [Electronic] Netherlands
• PMID: 21514285 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier B.V. All rights reserved.

Chemical References

• Anti-HIV Agents
• Dideoxynucleosides
• HCP5 long noncoding RNA, human
• RNA, Long Noncoding
• RNA, Untranslated
• abacavir

Topics

• Adolescent
• Adult
• Aged
• Alleles
• Anti-HIV Agents (adverse effects)
• Dideoxynucleosides (adverse effects)
• Drug Hypersensitivity (blood, genetics)
• Female
• Genotype
• Humans
• Major Histocompatibility Complex (genetics)
• Male
• Middle Aged
• Polymerase Chain Reaction
• Polymorphism, Single Nucleotide (genetics)
• Predictive Value of Tests
• RNA, Long Noncoding
• RNA, Untranslated
• Reference Values
• Reproducibility of Results
• Sensitivity and Specificity
• Young Adult