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Effects of thalidomide on long-term bone marrow cultures from patients with myelodysplastic syndromes: induction of IL-10 expression in the stromal layers.

Abstract
The purpose of this study was to investigate the in vitro effects of thalidomide on long-term bone marrow cultures from patients with myelodysplastic syndrome. We demonstrated that thalidomide induced an increase in granulocyte-macrophage colony forming unit numbers and in IL-10 expression. Thalidomide also promoted a slight increase in IL-6, IL-1β and TNF-α expression in the stromal layers. The numbers of erythroid burst forming units, the apoptosis rate of hematopoietic cells, and VEGF and TNF-α expression levels in culture supernatants were not modulated. Our results indicate a participation of thalidomide upon the hematopoietic microenvironment of patients with myelodysplastic syndromes, especially in the up regulation of IL-10.
AuthorsMariana Lazarini, Fabíola Traina, Sheila M Winnischofer, Fernando F Costa, Mary Luci S Queiroz, Sara T Olalla Saad
JournalLeukemia research (Leuk Res) Vol. 35 Issue 8 Pg. 1102-7 (Aug 2011) ISSN: 1873-5835 [Electronic] England
PMID21511336 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2011 Elsevier Ltd. All rights reserved.
Chemical References
  • Angiogenesis Inhibitors
  • Interleukin-1beta
  • Interleukin-6
  • RNA, Messenger
  • Tumor Necrosis Factor-alpha
  • VEGFA protein, human
  • Vascular Endothelial Growth Factor A
  • Interleukin-10
  • Thalidomide
Topics
  • Adult
  • Aged
  • Angiogenesis Inhibitors (therapeutic use)
  • Bone Marrow (drug effects)
  • Coculture Techniques
  • Colony-Forming Units Assay
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Hematopoietic Stem Cells
  • Humans
  • Interleukin-10 (genetics, metabolism)
  • Interleukin-1beta (genetics, metabolism)
  • Interleukin-6 (genetics, metabolism)
  • Male
  • Middle Aged
  • Myelodysplastic Syndromes (drug therapy, genetics, metabolism)
  • RNA, Messenger (genetics)
  • Reverse Transcriptase Polymerase Chain Reaction
  • Stromal Cells (drug effects, metabolism)
  • Thalidomide (therapeutic use)
  • Tumor Necrosis Factor-alpha (genetics, metabolism)
  • Vascular Endothelial Growth Factor A (genetics, metabolism)

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