[Cell continuous extraction-HPLC determination biological affinity of 8 bufadienolides on MGC-803 cells and their correlation with anti-tumor activities].

Abstract

OBJECTIVE:

To study the bioaffinity between 8 bufadienolides(Bu) and tumor cells and analyze the correlation between the bioaffinity and the anti-tumor activities of Bu.

METHOD:

Mix and cultivate the chloroform extract of Chansu and MGC-803. Measure the content of 8 Bu in supernatant and cells using HPLC and calculate their affinity rate.

RESULT:

The coefficient correlation between the decrease of Bu in cell supernatant after affinity and its MGC-803 restrictive activities, and between the cotent percentage of the free Bu in free cells with its MGC-803 restrictive activities, and between the difference between the decrease and the percentage and its MGC-803 restrictive activities is r = 0.82 (P < 0.05), r = -0.04 and r = 0.83 (P < 0.05) respectively.

CONCLUSION:

Eight Bu have different levels of affinity with MGC-803 which correlate with their anti-tumor activities.

• Authors: Jiejun Jiang, Fenqiang You, Hongyue Ma, Jing Zhong, Junfeng Zhang, Zhen Zhan, Yuping Tang, Jin'ao Duan, Anwei Ding
• Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (Zhongguo Zhong Yao Za Zhi) Vol. 36 Issue 2 Pg. 205-8 (Jan 2011) ISSN: 1001-5302 [Print] China
• PMID: 21506424 (Publication Type: Evaluation Study, Journal Article)

Chemical References

• Amphibian Venoms
• Antineoplastic Agents
• Bufanolides
• chan su

Topics

• Amphibian Venoms (chemistry)
• Animals
• Antineoplastic Agents (isolation & purification, pharmacology)
• Anura
• Bufanolides (isolation & purification, pharmacology)
• Cell Line, Tumor
• Chromatography, High Pressure Liquid (methods)
• Neoplasms (drug therapy)