Inoculation with Xanthomonas oryzae pv. oryzae induces thylakoid membrane association of Rubisco activase in Oryza meyeriana.

Abstract

Oryza meyeriana is a wild species of rice with high resistance to Xanthomonas oryzae pv. oryzae (Xoo), but the resistance mechanism is poorly understood. Protein gel blot analysis and immuno-gold electron microscopy showed that Xoo infection induced an association of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activase (RCA) with the thylakoid membrane in O. meyeriana, which led to considerable decline in the initial activity and the activation state of Rubisco. In susceptible cultivated rice, RCA remained in the chloroplast stroma. RCA may play a role in resistance to Xoo in O. meyeriana that differs from its well-known role in activating Rubisco, perhaps by protecting the thylakoid membrane against damage from Xoo.

• Authors: Yong Yang, Chu-Lang Yu, Xu-Ming Wang, Cheng-Qi Yan, Ye Cheng, Jian-Ping Chen
• Journal: Journal of plant physiology (J Plant Physiol) Vol. 168 Issue 14 Pg. 1701-4 (Sep 15 2011) ISSN: 1618-1328 [Electronic] Germany
• PMID: 21501886 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier GmbH. All rights reserved.

Chemical References

• Plant Proteins
• rca protein, plant

Topics

• Immunohistochemistry
• Microscopy, Immunoelectron
• Oryza (enzymology, immunology, microbiology, ultrastructure)
• Plant Diseases (immunology, microbiology)
• Plant Immunity
• Plant Leaves (enzymology, ultrastructure)
• Plant Proteins (metabolism)
• Thylakoids (enzymology, ultrastructure)
• Time Factors
• Xanthomonas (physiology)