[Effect and mechanisms of rutaecarpine on treating atopic dermatitis in mice].

Abstract

OBJECTIVE:

To study the effect of Rutaecarpine on the treatment of atopic dermatitis in mice.

METHODS:

DNCB was repeatedly applied on the back of NC/Nga mice to establish the animal model of atopic dermatitis. The mice without and with treatments with various does of Rutaecarpine were compared. Atopic dermatitis-like skin lesions were evaluated by skin histopathology and immunological parameters. The plasma IL-4, IgE and IFN-gamma were measured with enzyme-linked immunosorbent assay (ELISA).

RESULTS:

Topical DNCB induced eczematous dermatitis in Nc/Nga mice. The animal model of atopic dermatitis had higher levels of plasma IgE than the normal mice [(124.42 +/- 11.14) ng/mL vs. (17.22 +/- 3.56) ng/mL, P < 0.05]. The animal model of atopic dermatitis treated with Rutaecarpine had higher levels of plasma IFN-gamma level [(68.29 +/- 1.39) pg/mL] than those without treatment [(51.23 +/- 11.45) pg/mL]. The animal model of atopic dermatitis treated with Rutaecarpine had lower levels of plasma IL-4 and IgE [(72.11 +/- 2.13) pg/mL and (69.17 +/- 4.15) ng/mL, respectively] than those without treatment [(95.49 +/- 6.32) pg/mL and (124.42 +/- 11.14) ng/mL, respectively, P < 0.05]. No significant differences in plasma levels of IL-4, IFN-gamma and IgE were found between the mice treated with Rutaecarpine and those treated with Dexamethasone Acetate Cream [(76.14 +/- 3.63) pg/mL, (64.12 +/- 1.19) pg/mL, and (68.17 +/- 1.15) ng/mL, respectively, P > 0.05].

CONCLUSION:

The therapeutic effect of Rutaecarpine on atopic dermatitis-like skin lesions may be taken through inhibiting IgE and IL-4 synthesis and promoting the secretion of IFN-gamma.

• Authors: Min Tong, Ya-nan Guo, Gui-ying Zhang
• Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition (Sichuan Da Xue Xue Bao Yi Xue Ban) Vol. 42 Issue 2 Pg. 234-6, 263 (Mar 2011) ISSN: 1672-173X [Print] China
• PMID: 21500561 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Indole Alkaloids
• Quinazolines
• Interleukin-4
• Immunoglobulin E
• Interferon-gamma
• rutecarpine

Topics

• Animals
• Dermatitis, Atopic (drug therapy)
• Female
• Immunoglobulin E (blood)
• Indole Alkaloids (therapeutic use)
• Interferon-gamma (blood)
• Interleukin-4 (blood)
• Male
• Mice
• Quinazolines (therapeutic use)