[Expression of vigilin in cell lines and human hepatocellular carcinoma].

Abstract

OBJECTIVE:

To explore the expression status of Vigilin (high density lipoprotein binding protein) in various cell lines and human hepatocellular carcinoma (HCC) tissues.

METHODS:

The expression of Vigilin was measured semiquantitatively with western blot in hepatic cancer, cervical cancer and normal cell lines. The samples of 59 hepatocellular carcinoma tissues, 59 adjacent liver tissues and 33 distant non-tumor liver tissues were collected, Vigilin expression in the above samples was detected by immunohistochemistry.

RESULTS:

Vigilin expressed in all cell lines, but no expression was found in peripheral blood lymphocytes. The expression levels of Vigilin in tumor cell lines were higher than those in normal cell lines (P < 0.05). Most of the hepatic cells expressed Vigilin, but the expression levels were different (tumor tissues: 0.2226 +/- 0.054, adjacent tissues: 0.2060 +/- 0.056, distant tissues: 0.1820 +/- 0.038, P < 0.001). Highly expression of Vigilin was observed in 54% of tumor tissues, 35% adjacement tissues, and 6% of distant non-tumor tissues, respectively.

CONCLUSION:

Vigilin may have relationship with HCC progression and proliferation.

• Authors: Shu-wang Duan, Ling Wei, Wen-li Yang, Ke-fei Chen, Wen-qing Huang, Yang Qin
• Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition (Sichuan Da Xue Xue Bao Yi Xue Ban) Vol. 42 Issue 2 Pg. 170-3 (Mar 2011) ISSN: 1672-173X [Print] China
• PMID: 21500547 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• RNA-Binding Proteins
• high density lipoprotein binding protein

Topics

• Adult
• Aged
• Carcinoma, Hepatocellular (metabolism)
• Cell Line
• Cell Line, Tumor
• Female
• HeLa Cells
• Hep G2 Cells
• Humans
• Liver Neoplasms (metabolism)
• Male
• Middle Aged
• RNA-Binding Proteins (genetics, metabolism)