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Nanostructured delivery system for zinc phthalocyanine: preparation, characterization, and phototoxicity study against human lung adenocarcinoma A549 cells.

Abstract
In this study, zinc phthalocyanine (ZnPc) was loaded onto poly-ɛ-caprolactone (PCL) nanoparticles (NPs) using a solvent emulsification-evaporation method. The process yield and encapsulation efficiency were 74.2% ± 1.2% and 67.1% ± 0.9%, respectively. The NPs had a mean diameter of 187.4 ± 2.1 nm, narrow distribution size with a polydispersity index of 0.096 ± 0.004, zeta potential of -4.85 ± 0.21 mV, and spherical shape. ZnPc has sustained release, following Higuchi's kinetics. The photobiological activity of the ZnPc-loaded NPs was evaluated on human lung adenocarcinoma A549 cells. Cells were incubated with free ZnPc or ZnPc-loaded NPs for 4 h and then washed with phosphate-buffered saline. Culture medium was added to the wells containing the cells. Finally, the cells were exposed to red light (660 nm) with a light dose of 100 J/cm(2). The cellular viability was determined after 24 h of incubation. ZnPc-loaded NPs and free photosensitizer eliminated about 95.9% ± 1.8% and 28.7% ± 2.2% of A549 cells, respectively. The phototoxicity was time dependent up to 4 h and concentration dependent at 0-5 μg ZnPc. The cells viability decreased with the increase of the light dose in the range of 10-100 J/cm(2). Intense lysis was observed in the cells incubated with the ZnPcloaded NPs and irradiated with red light. ZnPc-loaded PCL NPs are the release systems that promise photodynamic therapy use.
AuthorsMariana da Volta Soares, Mainara Rangel Oliveira, Elisabete Pereira dos Santos, Lycia de Brito Gitirana, Gleyce Moreno Barbosa, Carla Holandino Quaresma, Eduardo Ricci-Júnior
JournalInternational journal of nanomedicine (Int J Nanomedicine) Vol. 6 Pg. 227-38 ( 2011) ISSN: 1178-2013 [Electronic] New Zealand
PMID21499420 (Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Indoles
  • Isoindoles
  • Organometallic Compounds
  • Photosensitizing Agents
  • Polyesters
  • Zinc Compounds
  • Zn(II)-phthalocyanine
  • polycaprolactone
Topics
  • Adenocarcinoma (drug therapy, metabolism, pathology)
  • Cell Line, Tumor
  • Cell Survival (drug effects)
  • Drug Delivery Systems
  • Humans
  • Indoles (administration & dosage, pharmacokinetics)
  • Isoindoles
  • Lung Neoplasms (drug therapy, metabolism, pathology)
  • Nanomedicine
  • Nanoparticles (administration & dosage, chemistry)
  • Organometallic Compounds (administration & dosage, pharmacokinetics)
  • Photochemotherapy (methods)
  • Photosensitizing Agents (administration & dosage, pharmacokinetics)
  • Polyesters (chemistry)
  • Zinc Compounds

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