Abstract |
In this study, zinc phthalocyanine (ZnPc) was loaded onto poly-ɛ- caprolactone (PCL) nanoparticles (NPs) using a solvent emulsification-evaporation method. The process yield and encapsulation efficiency were 74.2% ± 1.2% and 67.1% ± 0.9%, respectively. The NPs had a mean diameter of 187.4 ± 2.1 nm, narrow distribution size with a polydispersity index of 0.096 ± 0.004, zeta potential of -4.85 ± 0.21 mV, and spherical shape. ZnPc has sustained release, following Higuchi's kinetics. The photobiological activity of the ZnPc-loaded NPs was evaluated on human lung adenocarcinoma A549 cells. Cells were incubated with free ZnPc or ZnPc-loaded NPs for 4 h and then washed with phosphate-buffered saline. Culture medium was added to the wells containing the cells. Finally, the cells were exposed to red light (660 nm) with a light dose of 100 J/cm(2). The cellular viability was determined after 24 h of incubation. ZnPc-loaded NPs and free photosensitizer eliminated about 95.9% ± 1.8% and 28.7% ± 2.2% of A549 cells, respectively. The phototoxicity was time dependent up to 4 h and concentration dependent at 0-5 μg ZnPc. The cells viability decreased with the increase of the light dose in the range of 10-100 J/cm(2). Intense lysis was observed in the cells incubated with the ZnPcloaded NPs and irradiated with red light. ZnPc-loaded PCL NPs are the release systems that promise photodynamic therapy use.
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Authors | Mariana da Volta Soares, Mainara Rangel Oliveira, Elisabete Pereira dos Santos, Lycia de Brito Gitirana, Gleyce Moreno Barbosa, Carla Holandino Quaresma, Eduardo Ricci-Júnior |
Journal | International journal of nanomedicine
(Int J Nanomedicine)
Vol. 6
Pg. 227-38
( 2011)
ISSN: 1178-2013 [Electronic] New Zealand |
PMID | 21499420
(Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Indoles
- Isoindoles
- Organometallic Compounds
- Photosensitizing Agents
- Polyesters
- Zinc Compounds
- Zn(II)-phthalocyanine
- polycaprolactone
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Topics |
- Adenocarcinoma
(drug therapy, metabolism, pathology)
- Cell Line, Tumor
- Cell Survival
(drug effects)
- Drug Delivery Systems
- Humans
- Indoles
(administration & dosage, pharmacokinetics)
- Isoindoles
- Lung Neoplasms
(drug therapy, metabolism, pathology)
- Nanomedicine
- Nanoparticles
(administration & dosage, chemistry)
- Organometallic Compounds
(administration & dosage, pharmacokinetics)
- Photochemotherapy
(methods)
- Photosensitizing Agents
(administration & dosage, pharmacokinetics)
- Polyesters
(chemistry)
- Zinc Compounds
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