Abstract | PURPOSE: METHODS: Using a surgically induced model of myelomeningocele (MMC) in sheep, we studied the effects of different repair methods upon the development of hydrocephalus, the presence of the Arnold-Chiari II (AC-II) hindbrain malformation, and cerebral cortex developmental anomalies using gross and histologic ( hematoxylin and eosin and Nissl staining) study techniques. RESULTS: A malformed cerebral cortex, including 2 anomalous cortical folding patterns, and lower brain weights were observed in the untreated animals. Hydrocephalus and AC-II malformations were also found in this group. These malformations were mostly prevented with prenatal 2-layer closure. CONCLUSIONS: Cerebral cortical malformations and hydrocephalus, in addition to the AC-II hindbrain malformation, are disorders caused by fetal CSF leakage. These malformations were prevented with the technique of MMC closure currently used in humans. Both observations magnify the importance of the second hit associated with chronic CSF leakage, in addition to the primary defect causing the MMC, in the development of the malformation complex.
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Authors | Jose L Encinas, Miguel Ángel García-Cabezas, James Barkovich, César G Fontecha, Jose L Peiró, Garriboli M Carmen Soto, Victor Borrell, Isabel Reillo, Manuel López-Santamaría, Juan A Tovar, Diana L Farmer |
Journal | Journal of pediatric surgery
(J Pediatr Surg)
Vol. 46
Issue 4
Pg. 713-722
(Apr 2011)
ISSN: 1531-5037 [Electronic] United States |
PMID | 21496543
(Publication Type: Comparative Study, Journal Article)
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Copyright | Copyright © 2011 Elsevier Inc. All rights reserved. |
Topics |
- Animals
- Cerebral Cortex
(abnormalities, embryology, surgery)
- Disease Models, Animal
- Female
- Fetus
(pathology, surgery)
- Meningomyelocele
(embryology, surgery)
- Neurosurgical Procedures
(methods)
- Pregnancy
- Pregnancy, Animal
- Sheep
(embryology)
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