Supramolecular assemblies of rifampicin and cationic bilayers: preparation, characterization and micobactericidal activity.

Abstract

BACKGROUND:

Cationic bilayers based on the inexpensive synthetic lipid dioctadecyldimethylammonium bromide (DODAB) have been useful as carriers for drug delivery, immunoadjuvants for vaccines and active antimicrobial agents.

METHODS:

Rifampicin (RIF) or isoniazid (ISO) interacted with DODAB bilayer fragments (BF) or large vesicles (LV). Dispersions were evaluated by dynamic light-scattering for zeta-average diameter (Dz) and zeta-potential (ζ) analysis; dialysis for determination of drug entrapment efficiency; plating and CFU counting for determination of cell viability of Mycobacterium smegmatis or tuberculosis, minimal bactericidal concentration (MBC) and synergism index for DODAB/drug combinations.

RESULTS:

DODAB alone killed micobacteria over a range of micromolar concentrations. RIF aggregates in water solution were solubilised by DODAB BF. RIF was incorporated in DODAB bilayers at high percentiles in contrast to the leaky behavior of ISO. Combination DODAB/RIF yielded MBCs of 2/2 and 4/0.007 μg/mL against Mycobacterium smegmatis or Mycobacterium tuberculosis, respectively. Synergism indexes equal to 0.5 or 1.0, indicated synergism against the former and independent action, against the latter species.

CONCLUSIONS:

In vitro, DODAB acted effectively both as micobactericidal agent and carrier for rifampicin. The novel assemblies at reduced doses may become valuable against tuberculosis.

• Authors: Lilian Barbassa, Elsa M Mamizuka, Ana M Carmona-Ribeiro
• Journal: BMC biotechnology (BMC Biotechnol) Vol. 11 Pg. 40 (Apr 15 2011) ISSN: 1472-6750 [Electronic] England
• PMID: 21496246 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Bacterial Agents
• Anti-Infective Agents
• Antibiotics, Antitubercular
• Drug Carriers
• Lipid Bilayers
• Quaternary Ammonium Compounds
• dimethyldioctadecylammonium
• Rifampin

Topics

• Anti-Bacterial Agents (chemistry, pharmacology)
• Anti-Infective Agents (chemistry, pharmacology)
• Antibiotics, Antitubercular (chemistry, pharmacology)
• Drug Carriers
• Drug Compounding (methods)
• Drug Synergism
• Lipid Bilayers (chemistry)
• Microbial Sensitivity Tests
• Microbial Viability (drug effects)
• Molecular Structure
• Mycobacterium smegmatis (drug effects, growth & development)
• Mycobacterium tuberculosis (drug effects, growth & development)
• Quaternary Ammonium Compounds (chemistry, pharmacology)
• Rifampin (chemistry, pharmacology)
• Technology, Pharmaceutical (methods)