Effect of ciprofloxacin/dexamethasone versus ciprofloxacin/hydrocortisone on lipopolysaccharide-induced experimental otitis media.

Abstract

OBJECTIVES:

The purpose of this study is to compare the effect of topical ciprofloxacin/dexamethasone versus topical ciprofloxacin/hydrocortisone on the outcome of lipopolysaccharide (LPS)–induced otitis media with effusion in chinchillas.

STUDY DESIGN:

A randomized experimental animal study.

SETTING:

Jerry L. Pettis Veteran's Medical Center.

SUBJECTS AND METHODS:

Otitis media with effusion was induced in 5 groups of chinchillas, 6 per group, by injecting 0.3 mL (1 mg/mL) of Salmonella enteric LPS into the superior bullae of each chinchilla with a venting needle in place. Each group was treated with 0.2 mL of test substance at –2, 24, 48, and 72 hours relative to the 0-hour LPS induction. Group 1 was treated with vehicle control. Groups 2 to 5 received 0.3% ciprofloxacin with either 0.1% dexamethasone (group 2), 1% dexamethasone (group 3), 0.1% hydrocortisone (group 4), or 1% hydrocortisone (group 5). The outcome of each treatment was measured by the amount of middle ear effusion present and mucosal thickness at 120 hours posttreatment.

RESULTS:

Ciprofloxacin/dexamethasone 1% significantly (P = .0150) reduced middle ear effusion compared with control. Ciprofloxacin/dexamethasone 1% significantly reduced the mucosal thickness when compared with vehicle control (P = .0005), ciprofloxacin/dexamethasone 0.1% (P = .0240), and ciprofloxacin/hydrocortisone 0.1% (P = 1.00). Results also showed a dose-response effect between the ciprofloxacin/dexamethasone concentrations.

CONCLUSIONS:

This study demonstrated that treatment with a combination of topical ciprofloxacin and corticosteroid decreased the middle ear effusion when compared with the control group and that ciprofloxacin/dexamethasone suspension reduced the severity of LPS-induced experimental otitis media more than ciprofloxacin/hydrocortisone did.

• Authors: Piali Dattaray, Charles Pudrith, Mary Ann Nyc, Dusan Martin, You Hyun Kim, Patrick Jahng, You Sun Chung, G Michael Wall, Timothy Jung
• Journal: Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery (Otolaryngol Head Neck Surg) Vol. 145 Issue 2 Pg. 288-94 (Aug 2011) ISSN: 1097-6817 [Electronic] England
• PMID: 21493267 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Anti-Infective Agents
• Anti-Inflammatory Agents
• Lipopolysaccharides
• Ciprofloxacin
• Dexamethasone
• Hydrocortisone

Topics

• Administration, Topical
• Animals
• Anti-Infective Agents (administration & dosage)
• Anti-Inflammatory Agents (administration & dosage)
• Chinchilla
• Ciprofloxacin (administration & dosage, therapeutic use)
• Dexamethasone (administration & dosage)
• Disease Models, Animal
• Dose-Response Relationship, Drug
• Drug Therapy, Combination
• Female
• Hydrocortisone (administration & dosage)
• Lipopolysaccharides (toxicity)
• Male
• Mucous Membrane (drug effects, pathology)
• Otitis Media with Effusion (chemically induced, drug therapy, pathology)
• Periosteum (drug effects, pathology)
• Temporal Bone (pathology)
• Treatment Outcome