Abstract | OBJECTIVES: METHODS: Rats were fed with a high-fat/high- sucrose/high- cholesterol diet (20% sucrose, 10% animal oil, 1.0% bile salt and 2.5% cholesterol) (hereinafter defined as diabetic groups) or Purina Rodent Chow (NC group), the former was intervened with low dose streptozotocin (30 mg/kg) after feeding 1 month to make diabetic model. The NC group was intervened with citrate buffer and the diabetic rats were intervened with myriocin (0.3 mg/kg Qod) (MTD group) or just solvent (DC group) for 14 weeks. The EDVD, thickness of fatty deposition under endothelium, ceramide, PI3K/PKB/eNOS, NO and other vital parameters were measured after the rats sacrificed. RESULTS: In DC group, the ceramide contents in serum and aorta increased, the EDVD was impaired, the fatty deposition under endothelium increased, and the phosphorylation of PI3K/PKB/eNOS and NO release decreased all compared with the NC group (P<0.05). Compared with the DC group, the ceramide contents in MTD group decreased, the EDVD ameliorated, the fatty deposition diminished, and PI3K/PKB/eNOS phosphorylation and NO release (P<0.05) increased. CONCLUSIONS: After treated with myriocin, the EDVD in diabetic rats has been improved by increasing PI3K/PKB/eNOS phosphorylation and NO release, and meanwhile the atherosclerosis has reversed.
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Authors | Li Chun, Zhou Junlin, Wang Aimin, Li Niansheng, Chen Benmei, Lei Minxiang |
Journal | Diabetes research and clinical practice
(Diabetes Res Clin Pract)
Vol. 93
Issue 1
Pg. 77-85
(Jul 2011)
ISSN: 1872-8227 [Electronic] Ireland |
PMID | 21492950
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2011 Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
- Ceramides
- Fatty Acids, Monounsaturated
- Nitric Oxide
- thermozymocidin
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Topics |
- Animals
- Atherosclerosis
(blood, drug therapy, metabolism)
- Blotting, Western
- Ceramides
(blood, metabolism)
- Chromatography, Liquid
- Diabetes Mellitus, Experimental
(blood, drug therapy, metabolism)
- Endothelium, Vascular
(drug effects, pathology, ultrastructure)
- Fatty Acids, Monounsaturated
(therapeutic use)
- Immunohistochemistry
- Male
- Mass Spectrometry
- Microscopy, Electron, Transmission
- Nitric Oxide
(blood)
- Rats
- Rats, Sprague-Dawley
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