Abstract | BACKGROUND: METHODS AND RESULTS: AAs mixture was extracted with methanol from the defatted material of Aristolochia bracteolata whole plant at room temperature and was isolated from the aqueous methanol extract by chloroform. Moreover, Silica-gel column chromatography and Preparative Thin Layer Chromatography (PTLC) using chloroform/ methanol gradient mixtures were used to isolate AAs mixtures as a yellow crystalline solid. A preliminary detection of AAs was made by Thin Layer Chromatography ( silica-gel, chloroform: methanol (6:1)). The Rf value of the acids mixture was 0.43-0.46. The presence of AAs in plant sample was confirmed by High Performance Liquid Chromatography/Ultraviolet (HPLC/UV) analysis using 1% acetic acid and methanol (40:60) as mobile phase and maximum absorption wave length of 250 nm. Quantitative determination of AA-II (49.03 g/kg) and AA-I (12.98 g/kg) was also achieved by HPLC/UV. RECOMMENDATION: It is recommended that the use of Aristolochia bracteolata as a medicinal plant should be extremely limited or strictly prohibited. The chromatograms obtained in this study can serve as fingerprints to identify AAs in plant samples.
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Authors | Abdelgadir A Abdelgadir, Elhadi M Ahmed, Mahgoub Sharif Eltohami |
Journal | Environmental health insights
(Environ Health Insights)
Vol. 5
Pg. 1-8
(Feb 27 2011)
ISSN: 1178-6302 [Electronic] United States |
PMID | 21487531
(Publication Type: Journal Article)
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