Abstract | AIM: To explore the purification methods of wild-type and recombinant suilysin and to evaluate their biological activities. METHODS: RESULTS: Both prepared wild-type and recombinant suilysin, with purify over 90%, have hemolysis activity and could injure target cells at high concentration while cholesterol could completely inhibit their activities. CONCLUSION: Recombinant suilysin has similar biological activities with wild-type suilysin, and this work contributed to further study the functions of suilysin on pathogenesis of steptococcus suis.
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Authors | Qing-yu Lv, Huai-jie Hao, Li-li Bi, Yu-ling Zheng, Yong-qiang Jiang, Shu-xia Lv |
Journal | Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
(Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi)
Vol. 27
Issue 4
Pg. 374-6
(Apr 2011)
ISSN: 1007-8738 [Print] China |
PMID | 21481309
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Hemolysin Proteins
- Recombinant Proteins
- suilysin
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Topics |
- Hemolysin Proteins
(isolation & purification, pharmacology)
- Humans
- Recombinant Proteins
(isolation & purification, pharmacology)
- Serotyping
- Streptococcus suis
(chemistry, classification)
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