Evaluation of lytic activity of staphylococcal bacteriophage Sb-1 against freshly isolated clinical pathogens.

Abstract

In recent decades the increase in antibiotic-resistant bacterial strains has become a serious threat to the treatment of infectious diseases. Drug resistance of Staphylococcus aureus has become a major problem in hospitals of many countries, including developed ones. Today the interest in alternative remedies to antibiotics, including bacteriophage treatment, is gaining new ground. Here, we describe the staphylococcal bacteriophage Sb-1 - a key component of therapeutic phage preparation that was successfully used against staphylococcal infections during many years in the Former Soviet Union. This phage still reveals a high spectrum of lytic activity in vitro against freshly isolated, genetically different clinical samples (including methicillin-resistant S. aureus) obtained from the local hospitals, as well as the clinics from different geographical areas. The sequence analyses of phage genome showed absence of bacterial virulence genes. A case report describes a promising clinical response after phage application in patient with cystic fibrosis and indicates the efficacy of usage of Sb-1 phage against various staphylococcal infections.

• Authors: Leila Kvachadze, Nana Balarjishvili, Tamila Meskhi, Ekaterine Tevdoradze, Natia Skhirtladze, Tamila Pataridze, Revaz Adamia, Temur Topuria, Elizabeth Kutter, Christine Rohde, Mzia Kutateladze
• Journal: Microbial biotechnology (Microb Biotechnol) Vol. 4 Issue 5 Pg. 643-50 (Sep 2011) ISSN: 1751-7915 [Electronic] United States
• PMID: 21481199 (Publication Type: Case Reports, Evaluation Study, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: © 2011 The Authors. Journal compilation © 2011 Society for Applied Microbiology and Blackwell Publishing Ltd.

Chemical References

• Anti-Bacterial Agents
• Viral Proteins

Topics

• Anti-Bacterial Agents (pharmacology)
• Base Sequence
• Biological Therapy (methods)
• Child
• Drug Resistance, Bacterial
• Female
• Genome, Viral
• Humans
• Molecular Sequence Data
• Phylogeny
• Staphylococcal Infections (microbiology, therapy)
• Staphylococcus Phages (genetics, physiology)
• Staphylococcus aureus (classification, genetics, isolation & purification, virology)
• Viral Proteins (genetics, metabolism)