Raw starch fermentation to ethanol by an industrial distiller's yeast strain of Saccharomyces cerevisiae expressing glucoamylase and α-amylase genes.

Abstract

Industrial strains of a polyploid, distiller's Saccharomyces cerevisiae that produces glucoamylase and α-amylase was used for the direct fermentation of raw starch to ethanol. Strains contained either Aspergillus awamori glucoamylase gene (GA1), Debaryomyces occidentalis glucoamylase gene (GAM1) or D. occidentalis α-amylase gene (AMY), singly or in combination, integrated into their chromosomes. The strain expressing both GA1 and AMY generated 10.3% (v/v) ethanol (80.9 g l(-1)) from 20% (w/v) raw corn starch after 6 days of fermentation, and decreased the raw starch content to 21% of the initial concentration.

• Authors: Ha-Ram Kim, Young-Kum Im, Hyun-Mi Ko, Jong-Eon Chin, Il-Chul Kim, Hwanghee Blaise Lee, Suk Bai
• Journal: Biotechnology letters (Biotechnol Lett) Vol. 33 Issue 8 Pg. 1643-8 (Aug 2011) ISSN: 1573-6776 [Electronic] Netherlands
• PMID: 21479627 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Fungal Proteins
• Recombinant Proteins
• Ethanol
• Starch
• alpha-Amylases
• Glucan 1,4-alpha-Glucosidase

Topics

• Cloning, Molecular
• Debaryomyces (enzymology, genetics)
• Ethanol (analysis, metabolism)
• Fermentation
• Fungal Proteins (metabolism)
• Glucan 1,4-alpha-Glucosidase (biosynthesis, genetics, metabolism)
• Industrial Microbiology (methods)
• Recombinant Proteins (metabolism)
• Saccharomyces cerevisiae (genetics, metabolism)
• Starch (analysis, metabolism)
• alpha-Amylases (biosynthesis, genetics, metabolism)