Prognostic significance of cyclooxygenase-2 and response to chemotherapy in invasive ductal breast carcinoma patients by real time surface plasmon resonance analysis.

Abstract

Cyclooxygenase-2 (COX-2), an inducible enzyme, has been implicated in the progression and angiogenesis of breast cancer. The aim of the study is to quantify the concentration of COX-2 and its association with clinico-pathological parameters and response to treatment in patients with invasive ductal carcinoma receiving both neo-adjuvant and adjuvant chemotherapy. The level of COX-2 was estimated using a novel biosensor-based surface plasmon resonance technique in serum of 84 patients with breast cancer (48 patients of neo-adjuvant chemotherapy and 36 patients of adjuvant chemotherapy) and 40 age- and gender-matched normal individuals. A significant increase in COX-2 level was observed in patients compared with normal individuals (p>0.0001). The COX-2 level in serum was found to be significantly higher in patients with lymph node involvement (p<0.0061). 68% (33/48) of the patients receiving neo-adjuvant chemotherapy showed significantly (p<0.0025) reduced COX-2 levels. This study shows significant decrease of COX-2 level in patients with breast cancer treated with both neo-adjuvant and adjuvant chemotherapy. Estimation of COX-2 level in serum may serve as a tumor biomarker in patients with breast cancer.

• Authors: Abhay Kumar Singh, Rajinder Parshad, Shweta Pasi, Thirumurthy Madhavan, Satya N Das, Biswajit Mishra, Kamaldeep Gill, Krishna Dalal, Sharmistha Dey
• Journal: DNA and cell biology (DNA Cell Biol) Vol. 30 Issue 10 Pg. 801-7 (Oct 2011) ISSN: 1557-7430 [Electronic] United States
• PMID: 21476934 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Biomarkers, Tumor
• Cyclooxygenase 2
• PTGS2 protein, human

Topics

• Adult
• Aged
• Antineoplastic Agents (administration & dosage, therapeutic use)
• Biomarkers, Tumor (blood, genetics)
• Blotting, Western
• Breast Neoplasms (blood, diagnosis, mortality, pathology, therapy)
• Breast Neoplasms, Male (blood, diagnosis, mortality, pathology, therapy)
• Carcinoma, Ductal, Breast (blood, diagnosis, mortality, pathology, therapy)
• Chemotherapy, Adjuvant
• Cyclooxygenase 2 (blood, genetics)
• Disease-Free Survival
• Female
• Humans
• Male
• Middle Aged
• Neoadjuvant Therapy
• Neoplasm Invasiveness
• Prognosis
• Surface Plasmon Resonance (instrumentation, methods)