Abstract | OBJECTIVE: DESIGN: Expression of Tap-1 and Tapasin in different cell lines was analysed. CAL27 cells were treated with IFN-γ. Antigen from the treated cells was presented by DCs. Pulsed DC was then co-cultivated with CD8+ T lymphocyte to induce antigen specific cytotoxic lymphocytes (CTLs). The immune response elicited by CTLs against OSCC was observed. RESULTS: A significant lower expression of Tap-1 and Tapasin was observed in OSCC cell lines. IFN-γ exerted time-dependent effect for increasing the expression of these genes. Antigen from the treated CAL27 cells was presented by DCs. CTLs were induced and generated a strong immune response in vitro and in vivo. CONCLUSIONS: Tap-1 and Tapasin were downregulated in OSCC. IFN-γ increased the expression of these genes. Use of IFN-γ- antigen induced DCs could induce stronger immune response in vitro and in vivo.
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Authors | Han Wei, Pan Hongya, Jiang Linlin, Ali Mujiang, Wei Kuijie, Zou Duohong, Hu Qingang, Zhang Zhiyuan |
Journal | Archives of oral biology
(Arch Oral Biol)
Vol. 56
Issue 9
Pg. 891-8
(Sep 2011)
ISSN: 1879-1506 [Electronic] England |
PMID | 21474117
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2011 Elsevier Ltd. All rights reserved. |
Chemical References |
- ATP Binding Cassette Transporter, Subfamily B, Member 2
- ATP-Binding Cassette Transporters
- Epitopes
- Membrane Transport Proteins
- TAP1 protein, human
- tapasin
- Interferon-gamma
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Topics |
- ATP Binding Cassette Transporter, Subfamily B, Member 2
- ATP-Binding Cassette Transporters
(biosynthesis, genetics)
- Animals
- Antigen Presentation
- Carcinoma, Squamous Cell
(immunology)
- Cell Line, Tumor
(drug effects)
- Coculture Techniques
- Dendritic Cells
(drug effects, immunology)
- Epitopes
- Gene Expression Regulation, Neoplastic
- Humans
- Interferon-gamma
(immunology, pharmacology)
- Lymphocyte Activation
- Membrane Transport Proteins
(biosynthesis, genetics)
- Mice
- Mice, Nude
- Mouth Neoplasms
(immunology)
- T-Lymphocytes, Cytotoxic
(immunology)
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