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IFN-γ enhances the anti-tumour immune response of dendritic cells against oral squamous cell carcinoma.

AbstractOBJECTIVE:
To investigate the expression of antigen processing-1 (Tap-1) and Tapasin in oral squamous cell carcinoma (OSCC), and observe the immune response against OSCC by use of IFN-γ-antigen induced dendritic cells (DCs) in vitro and in vivo.
DESIGN:
Expression of Tap-1 and Tapasin in different cell lines was analysed. CAL27 cells were treated with IFN-γ. Antigen from the treated cells was presented by DCs. Pulsed DC was then co-cultivated with CD8+ T lymphocyte to induce antigen specific cytotoxic lymphocytes (CTLs). The immune response elicited by CTLs against OSCC was observed.
RESULTS:
A significant lower expression of Tap-1 and Tapasin was observed in OSCC cell lines. IFN-γ exerted time-dependent effect for increasing the expression of these genes. Antigen from the treated CAL27 cells was presented by DCs. CTLs were induced and generated a strong immune response in vitro and in vivo.
CONCLUSIONS:
Tap-1 and Tapasin were downregulated in OSCC. IFN-γ increased the expression of these genes. Use of IFN-γ-antigen induced DCs could induce stronger immune response in vitro and in vivo.
AuthorsHan Wei, Pan Hongya, Jiang Linlin, Ali Mujiang, Wei Kuijie, Zou Duohong, Hu Qingang, Zhang Zhiyuan
JournalArchives of oral biology (Arch Oral Biol) Vol. 56 Issue 9 Pg. 891-8 (Sep 2011) ISSN: 1879-1506 [Electronic] England
PMID21474117 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2011 Elsevier Ltd. All rights reserved.
Chemical References
  • ATP Binding Cassette Transporter, Subfamily B, Member 2
  • ATP-Binding Cassette Transporters
  • Epitopes
  • Membrane Transport Proteins
  • TAP1 protein, human
  • tapasin
  • Interferon-gamma
Topics
  • ATP Binding Cassette Transporter, Subfamily B, Member 2
  • ATP-Binding Cassette Transporters (biosynthesis, genetics)
  • Animals
  • Antigen Presentation
  • Carcinoma, Squamous Cell (immunology)
  • Cell Line, Tumor (drug effects)
  • Coculture Techniques
  • Dendritic Cells (drug effects, immunology)
  • Epitopes
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Interferon-gamma (immunology, pharmacology)
  • Lymphocyte Activation
  • Membrane Transport Proteins (biosynthesis, genetics)
  • Mice
  • Mice, Nude
  • Mouth Neoplasms (immunology)
  • T-Lymphocytes, Cytotoxic (immunology)

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