Abstract | OBJECTIVES: STUDY DESIGN: A prospective study of tissues obtained prospectively from 28 women with laparoscopically confirmed minimal to moderate endometriosis treated with LNG-IUS for 6 months. Endometrial and endometriotic biopsies obtained before and 6 months after treatment were processed and stained for ER-α, ER-β and PR expression by immunohistochemistry. Photographs were obtained and the receptors quantified. RESULTS: The mean (±SD) age of the 28 women was 31±7.2 (range 18-42) years. Eight of them at initial biopsy were in the proliferative phase and 20 in the secretory phase. ER-α, ER-β and PR expression decreased significantly in the glandular (P<0.0001) and stromal (P<0.0001) compartments of the eutopic endometrium after treatment with LNG-IUS. Similarly, ER-α, ER-β and PR were significantly decreased in the stromal compartment of ectopic endometrium (P<0.0001), and significantly decreased in the ectopic glands of ER-α (P<0.0001), ER-β (P=0.0002) and PR (P=0.0064) expression. CONCLUSION: The ameliorative effect of LNG-IUS on the symptoms of minimal to moderate endometriosis is likely modulated through a decrease in the expression of glandular and stromal ER-α, ER-β and PR in the ectopic endometrium.
|
Authors | Samuel L Engemise, Jonathon M Willets, Anthony H Taylor, Joseph O Emembolu, Justin C Konje |
Journal | European journal of obstetrics, gynecology, and reproductive biology
(Eur J Obstet Gynecol Reprod Biol)
Vol. 157
Issue 1
Pg. 101-6
(Jul 2011)
ISSN: 1872-7654 [Electronic] Ireland |
PMID | 21470761
(Publication Type: Clinical Trial, Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright © 2011 Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
- Protein Isoforms
- Receptors, Estrogen
- Receptors, Progesterone
- Levonorgestrel
|
Topics |
- Adolescent
- Adult
- Biopsy
- Down-Regulation
(drug effects)
- Drug Delivery Systems
- Endometriosis
(drug therapy, metabolism, pathology)
- Endometrium
(drug effects, metabolism, pathology)
- Female
- Follicular Phase
(metabolism)
- Humans
- Immunohistochemistry
- Levonorgestrel
(administration & dosage, therapeutic use)
- Luteal Phase
(metabolism)
- Protein Isoforms
(metabolism)
- Receptors, Estrogen
(metabolism)
- Receptors, Progesterone
(metabolism)
- Severity of Illness Index
- Young Adult
|