Polyozellin blocks tumor necrosis factor α-induced interleukin 8 and matrix metalloproteinase 7 production in the human intestinal epithelial cell line HT-29.

Abstract

Polyozellin isolated from Polyozellus multiplex (Thelephoraceae) displays potent anti-inflammatory effects in murine macrophages. Here we evaluated whether polyozellin has the potential to ameliorate diseases characterized by mucosal inflammation in intestinal epithelial HT-29 cells. Polyozellin significantly inhibited tumor necrosis factor (TNF)-α-induced interleukin-8 secretion and mRNA expression. Moreover, polyozellin suppressed the expression of matrix metalloproteinase-7 mRNA and extracellular pro-matrix metalloproteinase-7 secretion. A signal transduction study revealed that polyozellin significantly attenuates TNF-α-mediated p38 phosphorylation, inhibitory factor κBα degradation, and nuclear factor-κB-mediated transcriptional activation. Collectively, these results suggest that polyozellin has the potential to attenuate intestinal inflammation and shed light on the novel signal pathway evoked by TNF-α during intestinal inflammation.

• Authors: Sung Hee Lee, Kyung-Sik Song, Dong Hwan Sohn, Geom Seog Seo
• Journal: Archives of pharmacal research (Arch Pharm Res) Vol. 34 Issue 1 Pg. 91-7 (Jan 2011) ISSN: 1976-3786 [Electronic] Korea (South)
• PMID: 21468920 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Furans
• I-kappa B Proteins
• Interleukin-8
• NF-kappa B
• NFKBIA protein, human
• RNA, Messenger
• Tumor Necrosis Factor-alpha
• polyozellin
• NF-KappaB Inhibitor alpha
• p38 Mitogen-Activated Protein Kinases
• Matrix Metalloproteinase 7

Topics

• Basidiomycota (chemistry)
• Furans (isolation & purification, pharmacology)
• HT29 Cells
• Humans
• I-kappa B Proteins (drug effects, metabolism)
• Inflammation (drug therapy, physiopathology)
• Interleukin-8 (drug effects, metabolism)
• Intestinal Mucosa (drug effects, pathology)
• Matrix Metalloproteinase 7 (drug effects, metabolism)
• NF-KappaB Inhibitor alpha
• NF-kappa B (drug effects, metabolism)
• Phosphorylation (drug effects)
• RNA, Messenger (metabolism)
• Signal Transduction (drug effects)
• Tumor Necrosis Factor-alpha (antagonists & inhibitors)
• p38 Mitogen-Activated Protein Kinases (drug effects, metabolism)