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Differentiation of foot-and-mouth disease-infected pigs from vaccinated pigs using antibody-detecting sandwich ELISA.

Abstract
The presence of serum antibodies for nonstructural proteins of the foot-and-mouth disease virus (FMDV) can differentiate FMDV-infected animals from vaccinated animals. In this study, a sandwich ELISA was developed for rapid detection of the foot-and-mouth disease (FMD) antibodies; it was based on an Escherichia coli-expressed, highly conserved region of the 3ABC nonstructural protein of the FMDV O/TW/99 strain and a monoclonal antibody derived from the expressed protein. The diagnostic sensitivity of the assay was 98.4%, and the diagnostic specificity was 100% for naïve and vaccinated pigs; the detection ability of the assay was comparable those of the PrioCHECK and UBI kits. There was 97.5, 93.4 and 66.6% agreement between the results obtained from our ELISA and those obtained from the PrioCHECK, UBI and CHEKIT kits, respectively. The kappa statistics were 0.95, 0.87 and 0.37, respectively. Moreover, antibodies for nonstructural proteins of the serotypes A, C, Asia 1, SAT 1, SAT 2 and SAT 3 were also detected in bovine sera. Furthermore, the absence of cross-reactions generated by different antibody titers against the swine vesicular disease virus and vesicular stomatitis virus (VSV) was also highlighted in this assay's specificity.
AuthorsTsu-Han Chen, Fan Lee, Yeou-Liang Lin, Aldo Dekker, Wen-Bin Chung, Chu-Hsiang Pan, Ming-Hwa Jong, Chin-Cheng Huang, Ming-Chang Lee, Hsiang-Jung Tsai
JournalThe Journal of veterinary medical science (J Vet Med Sci) Vol. 73 Issue 8 Pg. 977-84 (Aug 2011) ISSN: 1347-7439 [Electronic] Japan
PMID21467761 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • 3ABC protein, virus
  • Antibodies, Viral
  • Viral Nonstructural Proteins
Topics
  • Animals
  • Antibodies, Viral (blood)
  • Cross Reactions
  • Enzyme-Linked Immunosorbent Assay (methods, veterinary)
  • Foot-and-Mouth Disease (diagnosis, prevention & control)
  • Foot-and-Mouth Disease Virus (immunology)
  • Sensitivity and Specificity
  • Swine
  • Swine Diseases (diagnosis, prevention & control)
  • Vaccination (veterinary)
  • Viral Nonstructural Proteins (blood)

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