Identification of trans-tiliroside as active principle with anti-hyperglycemic, anti-hyperlipidemic and antioxidant effects from Potentilla chinesis.

Abstract

AIM OF THE STUDY:

The present study was carried out to isolate and identify trans-tiliroside as principal compound with anti-hyperglycemic, anti-hyperlipidemic and antioxidant effects from Potentilla chinesis.

MATERIALS AND METHODS:

A bioactive compound, trans-tiliroside was isolated from the ethanol extract of Potentilla chinesis and its administration dose was optimized and patented. The normal, alloxan-induced diabetic mice and streptozotocin-induced diabetic rats were used to evaluate the anti-hyperglycemic, anti-hyperlipidemic and antioxidant effects of trans-tiliroside from Potentilla chinesis. Biochemical parameters were assayed in blood samples of different groups of alloxan-induced diabetic mice and streptozotocin-induced diabetic rats.

RESULTS:

The level of fasting serum glucose levels, triglycerides (TG) and total cholesterol (TC) in alloxan-induced diabetic mice were significantly decrease after daily oral administration of trans-tiliroside in normal and diabetic mice at doses of 0.4, 0.8 and 1.6 mg/kg/day, for 15 days. Blood glucose level was significant decrease in STZ induced diabetic rats by trans-tiliroside (1.2 and 0.3mg/kg body weight for 10 weeks). The content of TC, low density lipoprotein (LDL-C) and TG levels were decreased and high density lipoprotein (HDL-C) content was increased, so lipid metabolism was improved. Moreover, trans-tiliroside revealed antioxidant activity as shown by increased activities of superoxide dismutase (SOD), decreased level of malondialdehyde (MDA) in diabetic rats. Histological morphology examination showed that the trans-tiliroside restored the damage of pancreas tissues in rats with diabetes mellitus.

CONCLUSION:

Trans-tiliroside, a constituent from Potentilla chinesis, revealed significant anti-hyperglycemic, anti-hyperlipidemic and antioxidant activities.

• Authors: Wei Qiao, Chuan Zhao, Nan Qin, Hui Yuan Zhai, Hong Quan Duan
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 135 Issue 2 Pg. 515-21 (May 17 2011) ISSN: 1872-7573 [Electronic] Ireland
• PMID: 21463674 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Antioxidants
• Blood Glucose
• Flavonoids
• Hypoglycemic Agents
• Hypolipidemic Agents
• tiliroside
• Streptozocin
• Alloxan

Topics

• Alloxan
• Animals
• Antioxidants (pharmacology)
• Blood Glucose (analysis)
• Diabetes Mellitus, Experimental (blood)
• Dose-Response Relationship, Drug
• Flavonoids (administration & dosage, isolation & purification, pharmacology)
• Hypoglycemic Agents (pharmacology)
• Hypolipidemic Agents (pharmacology)
• Male
• Mice
• Potentilla (chemistry)
• Rats
• Rats, Sprague-Dawley
• Streptozocin