Abstract | ETHNOPHARMACOLOGICAL RELEVANCE: AIM OF THE STUDY: HRSV is a major pediatric viral pathogen of low respiratory tract infection without effective management. This study tested the hypothesis that SMGGT effectively inhibited cytopathy induced by HRSV. MATERIALS AND METHODS: Effect of the crude extract of SMGGT on HRSV was tested by plaque reduction assay in both human upper (HEp-2) and low (A549) respiratory tract cell lines. Ability of SMGGT to stimulate anti-viral cytokines was evaluated by enzyme-linked immunosorbent assay (ELISA). RESULTS:
Crude extract of SMGGT dose-dependently inhibited HRSV-induced plaque formation. The crude extract was more effective when given before viral infection (p<0.0001). It inhibited viral attachment dose-dependently (p<0.0001) and could increase heparin effect on viral attachment. Furthermore, it was synergistic with very low-dose heparin on viral attachment. In addition, the crude extract time-dependently and dose-dependently (p<0.0001) inhibited HRSV internalization into HEp-2 cells. Epithelial cells secrete IFN-β and TNF-α to counteract viral infection. The crude extract could stimulate epithelial cells to secrete these cytokines beforehand and become resistant to viral infection. It also stimulated IFN-β to defense HRSV after viral inoculation. CONCLUSIONS:
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Authors | Kuo-Chih Wang, Jung-San Chang, Lien-Chai Chiang, Chun-Ching Lin |
Journal | Journal of ethnopharmacology
(J Ethnopharmacol)
Vol. 135
Issue 2
Pg. 538-44
(May 17 2011)
ISSN: 1872-7573 [Electronic] Ireland |
PMID | 21463671
(Publication Type: Journal Article)
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Copyright | Copyright © 2011 Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
- Drugs, Chinese Herbal
- sheng-ma-ge-gen-tang
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Topics |
- Cell Line
- Cytopathogenic Effect, Viral
(drug effects)
- Drugs, Chinese Herbal
(pharmacology)
- Humans
- Medicine, Chinese Traditional
- Respiratory Syncytial Viruses
(drug effects, pathogenicity)
- Respiratory System
(cytology, virology)
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