Interleukin 13 receptors as biochemical markers in atopic patients.

Abstract

BACKGROUND:

Interleukin (IL) 13, a type 2 helper T cell (T(H)2), is an important regulator of inflammatory immune responses. It mediates its action through a receptor complex consisting of IL-13Ralpha1 and IL-4Ralpha. IL-13Ralpha2 binds IL-13 with high affinity and is thought to act primarily as a decoy receptor, sequestering IL-13 and thus inhibiting its action. Our aim was to clarify the role of these receptors in the diagnosis and follow-up of atopic patients.

METHODS:

We genotyped the 1398A>G polymorphism in the IL-13Ralpha1 gene using restriction fragment length polymorphism for causal genetic diversity and measured serum levels of IL-13Ralpha2 in 105 atopic patients suffering from atopic asthma, atopic dermatitis, and atopic rhinitis (35 each). We compared the results with those of 35 nonatopic control individuals. Total immunoglobulin (Ig) E and serum IL-13Ralpha2 were measured using enzyme-linked immunosorbent assay, and the eosinophil counts were recorded.

RESULTS:

A significant increase in serum IL-13Ralpha2 levels was recorded in the 3 atopic groups compared with the control group (P < .001), as well as a significant increase in total IgE levels and eosinophil counts. No significant association was found between 1398A>G and atopy other than a suggestive association between this polymorphism and raised total serum IgE levels in all 3 atopic groups (P < .001).

CONCLUSIONS:

These findings indicate that IL-13Ralpha2 plays an important role in atopy and that increased levels in different groups highlight its regulatory role in the development of atopic symptoms. The 1398A>G polymorphism might be involved in the production of IgE.

• Authors: Y M Hussein, A S Ahmad, M M Ibrahem, H M Elsherbeny, S M Shalaby, A S El-Shal, N A Sabbah
• Journal: Journal of investigational allergology & clinical immunology (J Investig Allergol Clin Immunol) Vol. 21 Issue 2 Pg. 101-7 ( 2011) ISSN: 1018-9068 [Print] Spain
• PMID: 21462799 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers
• Receptors, Interleukin-13
• Immunoglobulin E

Topics

• Asthma (blood, genetics, immunology)
• Biomarkers (analysis)
• Case-Control Studies
• Dermatitis, Atopic (blood, genetics, immunology)
• Eosinophils (cytology)
• Female
• Genotype
• Humans
• Immunoglobulin E (blood)
• Leukocyte Count
• Polymorphism, Genetic
• Receptors, Interleukin-13 (blood, genetics, immunology)
• Rhinitis (blood, genetics, immunology)