Isotropic micropatterned silica coatings on zirconia induce guided cell growth for dental implants.

Abstract

UNLABELLED:

Titanium implants are the gold standard in dentistry; however, problems such as gingival tarnishing and peri-implantitis have been reported. For zirconia to become a competitive alternative dental implant material, surface modification techniques that induce guided tissue growth must be developed.

OBJECTIVES:

To develop alternative surface modification techniques to promote guided tissue regeneration on zirconia materials, for applications in dental implantology.

METHODS:

A methodology that combined soft lithography and sol-gel chemistry was used to obtain isotropic micropatterned silica coatings on yttria-stabilized zirconia substrates. The materials were characterized via chemical, structural, surface morphology approaches. In vitro biological behavior was evaluated in terms of early adhesion and viability/metabolic activity of human osteoblast-like cells. Statistical analysis was conducted using one-way ANOVA/Tukey HSD post hoc test.

RESULTS:

Isotropic micropatterned silica coatings on yttria-stabilized zirconia substrates were obtained using a combined approach based on sol-gel technology and soft lithography. Micropatterned silica surfaces exhibited a biocompatible behavior, and modulated cell responses (i.e. inducing early alignment of osteoblast-like cells). After 7d of culture, the cells fully covered the top surfaces of pillar microstructured silica films.

SIGNIFICANCE:

The micropatterned silica films on zirconia showed a biocompatible response, and were capable of inducing guided osteoblastic cell adhesion, spreading and propagation. The results herein presented suggest that surface-modified ceramic implants via soft lithography and sol-gel chemistry could potentially be used to guide periodontal tissue regeneration, thus promoting tight tissue apposition, and avoiding gingival retraction and peri-implantitis.

• Authors: A Pelaez-Vargas, D Gallego-Perez, M Magallanes-Perdomo, M H Fernandes, D J Hansford, A H De Aza, P Pena, F J Monteiro
• Journal: Dental materials : official publication of the Academy of Dental Materials (Dent Mater) Vol. 27 Issue 6 Pg. 581-9 (Jun 2011) ISSN: 1879-0097 [Electronic] England
• PMID: 21459429 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
• Copyright: Copyright © 2011 Academy of Dental Materials. All rights reserved.

Chemical References

• Coated Materials, Biocompatible
• Dental Implants
• Dental Materials
• Indicators and Reagents
• Oxazines
• Xanthenes
• yttria stabilized tetragonal zirconia
• resazurin
• Yttrium
• Silicon Dioxide
• Zirconium

Topics

• Cell Adhesion (physiology)
• Cell Line
• Cell Proliferation
• Cell Survival (physiology)
• Ceramics (chemistry)
• Coated Materials, Biocompatible (chemistry)
• Crystallography
• Dental Implants
• Dental Materials (chemistry)
• Guided Tissue Regeneration, Periodontal
• Hot Temperature
• Humans
• Indicators and Reagents
• Materials Testing
• Microscopy, Confocal
• Microscopy, Electron, Scanning
• Osteoblasts (physiology)
• Oxazines
• Phase Transition
• Silicon Dioxide (chemistry)
• Spectrum Analysis, Raman
• Surface Properties
• Time Factors
• X-Ray Diffraction
• Xanthenes
• Yttrium (chemistry)
• Zirconium (chemistry)