Therapeutic ultrasonic microbubbles carrying paclitaxel and LyP-1 peptide: preparation, characterization and application to ultrasound-assisted chemotherapy in breast cancer cells.

Abstract

The aim of this work was to develop a novel targeted drug-loaded microbubble (MB) and to investigate its chemotherapy effect in vitro. Paclitaxel (PTX)-loaded lipid MBs were prepared by a mechanical vibration technique. The LyP-1, a breast tumor homing peptide, was coated onto the surface of PTX-loaded MBs through biotin-avidin linkage. The resulting targeted drug-loaded MBs were characterized and applied to ultrasound-assisted chemotherapy in breast cancer cells. Our results showed the ultrasonic MBs were able to achieve 43%-63% of drug encapsulation efficiency, depending on drug loading amount. The binding affinity assay indicated the attachment of targeted MBs to human MDA-MB-231 breast cancer cells was highly efficient and stable even with ultrasonic irradiation on. The cellular uptake efficiency of payload in targeted MBs was 3.71-, 4.95-, 7.43- and 7.66-fold higher than that of non-targeted MBs at the applied ultrasound time of 30, 60, 90 and 120 s, respectively. In addition, the cell proliferation inhibition assay showed the cell viability of targeted PTX-loaded MBs was significantly lower than that of non-targeted PTX-loaded MBs and non-targeted unloaded MBs when ultrasound was utilized. In conclusion, the study indicated the LyP-1-coated PTX-loaded MBs significantly increased the antitumor efficacy and can be used as a potential chemotherapy approach for ultrasound-assisted breast cancer treatment.

• Authors: Fei Yan, Xiang Li, Qiaofeng Jin, Chunxiang Jiang, Zidong Zhang, Tao Ling, Bensheng Qiu, Hairong Zheng
• Journal: Ultrasound in medicine & biology (Ultrasound Med Biol) Vol. 37 Issue 5 Pg. 768-79 (May 2011) ISSN: 1879-291X [Electronic] England
• PMID: 21458148 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 World Federation for Ultrasound in Medicine & Biology. Published by Elsevier Inc. All rights reserved.

Chemical References

• LyP-1 peptide
• Peptides, Cyclic
• Paclitaxel

Topics

• Breast Neoplasms (diagnostic imaging, drug therapy)
• Cell Line, Tumor
• Cell Survival (drug effects)
• Drug Delivery Systems
• Female
• Humans
• In Vitro Techniques
• Microbubbles (therapeutic use)
• Paclitaxel (therapeutic use)
• Peptides, Cyclic (therapeutic use)
• Ultrasonics
• Ultrasonography, Mammary